Rabbit PAK4 Polyclonal Antibody | anti-PAK4 antibody

PAK4 Antibody

Cat. Num. AAA841190

• Reactivity: Human, Mouse, Rat, Hamster
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: PAK4; Polyclonal Antibody; PAK4 Antibody; PAK-4; KIAA1142; anti-PAK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-PAK4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 591

• Applicable Applications for anti-PAK4 antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~60-70 kDa Pak4 in samples of human, mouse, and rat origins. The antibody does not cross-react with other Paks. Reactivity to other species has not been tested.
• Immunogen: Synthetic peptide corresponding to the N-terminus of human Pak4
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-PAK4 antibody

Background: The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region. It has been demonstrated that phosphorylation of serine 474 of PAK4 may play a pivotal role in the activity and function of PAK4 kinase

NCBI and Uniprot Product Information

• NCBI GI #: 158257110
• Molecular Weight: 63,966 Da
• NCBI Official Full Name: unnamed protein product
• UniProt Protein Name: Non-specific serine/threonine protein kinase
• Protein Family: Serine/threonine-protein kinase
• UniProt Entry Name: A8K724_HUMAN

NCBI Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: Golgi apparatus; focal adhesion

Molecular Function: protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; cell motility; signal transduction; cell growth; protein amino acid phosphorylation