Rabbit PAK4 (non-phospho Polyclonal Antibody | anti-PAK4 (non-phospho antibody

Rabbit anti PAK4 Polyclonal antibody

Cat. Num. AAA462101

• Reactivity: Human, Rat, Mouse, Bovine, Chicken, Canine
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: PAK4 (non-phospho; Polyclonal Antibody; Rabbit anti PAK4 Polyclonal antibody; Rabbit anti-PAK4; p21-activated kinase 4; anti-PAK4 (non-phospho antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Bovine, Chicken, Canine
• Clonality: Polyclonal
• Specificity: This antibody recognizes PAK4 protein. It does not cross react to other PAK1, PAK2 or PAK3. This antibody reacts with human, rat and mouse. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-PAK4 (non-phospho antibody: ELISA, Western Blot, Immunoprecipitation
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide derived from internal sequence of human PAK4. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
• Positive Control: HEK293
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-PAK4 (non-phospho antibody

p21-activated protein kinase (PAK) serine/threonine kinases are important effectors of Rho family GTPases and have been implicated in the regulation of cell morphology and motility, as well as in cell transformation. The phosphorylation of serine 474 as the likely autophosphorylation site in the kinase domain of PAK4 occurs in vivo. PAK4 is frequently over-expressed in human tumor cell lines of various tissue origins, indicates that the phosphorylation of PAK4 is required in oncogenic transformation and Ras-driven, anchorage-independent growth.

References

Marinella G. Callow et al. Requirement for PAK4 in the Anchorage-independent Growth of Human Cancer Cell Lines. (2002) J. Biol. Chem. 277 (1), 550-558.

NCBI and Uniprot Product Information

• NCBI GI #: 4164385
• NCBI GeneID: 10298
• UniProt Accession #: O96013; Q8N4E1; Q8NCH5; Q8NDE3; Q9BU33; Q9ULS8; B4DGG6
• Molecular Weight: 68 kDa
• NCBI Official Full Name: PAK4 protein
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 4
• NCBI Official Symbol: PAK4
• NCBI Protein Information: serine/threonine-protein kinase PAK 4; PAK-4; p21-activated kinase 4; p21(CDKN1A)-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• UniProt Protein Name: Serine/threonine-protein kinase PAK 4
• UniProt Gene Name: PAK4
• UniProt Synonym Gene Names: KIAA1142
• UniProt Entry Name: PAK4_HUMAN

NCBI Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts with FGFR2 and GRB2

By similarity. Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1. Interacts with its substrate ARHGEF2. Ref.9

Tissue specificity: Highest expression in prostate, testis and colon.

Post-translational modification: Autophosphorylated on serine residues when activated by CDC42/p21. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17Phosphorylated on tyrosine residues upon stimulation of FGFR2

By similarity. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain.

Sequence caution: The sequence BAA86456.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11166.1 differs from that shown. Reason: Erroneous initiation.