Rabbit TMPRSS2 Antibody | anti-TMPRSS2 antibody

TMPRSS2

Cat. Num. AAA9126790

• Gene Names: TMPRSS2; PP9284; PRSS10
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: TMPRSS2; PP9284; PRSS10; anti-TMPRSS2 antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-TMPRSS2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-400 of human TMPRSS2 (NP_001128571.1).
• Catagory: Primary Antibody
• Species Reactivity/ Cross Reactivity: Human, Mouse, Rat
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TMPRSS2 antibody (MBS9126790) at 1:1000dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 30s)

Western BLot (WB)

(Western blot analysis of extracts of various cell lines, using TMPRSS2 antibody (MBS9126790) at 1:500dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 180s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of parrafin-embedded human colon carcinoma using TMPRSS2 Rabbit pAb (MBS9126790) at dilution of 1:50 (40X lens))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded human placenta using TMPRSS2 Rabbit pAb (MBS9126790) at dilution of 1:50 (40x lens))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin embedded mouse lung using TMPRSS2 Rabbit pAb (MBS9126790) at dilution of 1:50 (40xlens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of A-549 cells using TMPRSS2 antibody (MBS9126790) at dilution of 1:50. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using TMPRSS2 antibody (MBS9126790) at dilution of 1:50. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using TMPRSS2 antibody (MBS9126790) at dilution of 1:50. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells using TMPRSS2 antibody (MBS9126790) at dilution of 1:50. Blue: DAPI for nuclear staining.)

Related Product Information for anti-TMPRSS2 antibody

Background: This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TMPRSS2 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 115502469
• NCBI GeneID: 7113
• UniProt Accession #: O15393; Q6GTK7; Q9BXX1; A8K6Z8; B2R8E5; B7Z459; D3DSJ2; F8WES1
• Molecular Weight: Observed MW: 70kDa/54kDa/32kDa; Calculated MW: 53kDa/57kDa
• NCBI Official Full Name: Transmembrane protease serine 2
• NCBI Official Synonym Full Names: transmembrane protease, serine 2
• NCBI Official Symbol: TMPRSS2
• NCBI Official Synonym Symbols: PP9284; PRSS10
• NCBI Protein Information: transmembrane protease serine 2; epitheliasin; serine protease 10
• UniProt Protein Name: Transmembrane protease serine 2
• Protein Family: Transmembrane protease serine
• UniProt Gene Name: TMPRSS2
• UniProt Synonym Gene Names: PRSS10
• UniProt Entry Name: TMPS2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TMPRSS2: a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Protein type: Protease; Membrane protein, integral; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; protein autoprocessing; positive regulation of virion penetration into host cell; proteolysis