Rabbit anti-Human SMAC Polyclonal Antibody | anti-DIABLO antibody

Anti-SMAC Antibody, Rabbit Polyclonal

Cat. Num. AAA8102357

• Gene Names: DIABLO; SMAC; SMAC3; DFNA64; DIABLO-S
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: SMAC; Polyclonal Antibody; Anti-SMAC Antibody; Rabbit Polyclonal; SMAC/Diablo Antibody; Rabbit PAb; diablo; IAP-binding mitochondrial protein; Anti-DFNA64 Antibody; anti-DIABLO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SMAC/Diablo
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DIABLO antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human Diablo protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Diablo (rh Diablo). Total IgG was purified by Protein A affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Diablo rabbit polyclonal antibody at 1:1000 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:20 kDa)

Related Product Information for anti-DIABLO antibody

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

Product Categories/Family for anti-DIABLO antibody

Death Receptor Signaling

References

Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.

NCBI and Uniprot Product Information

• NCBI GI #: 9845297
• NCBI GeneID: 56616
• NCBI Accession #: NP_063940.1
• NCBI GenBank Nucleotide #: NM_019887.4
• UniProt Accession #: Q9NR28; Q6W3F3; Q96LV0; Q9BT11; Q9HAV6; B2RDQ0
• Molecular Weight: 27,131 Da
• NCBI Official Full Name: diablo homolog, mitochondrial isoform 1
• NCBI Official Synonym Full Names: diablo, IAP-binding mitochondrial protein
• NCBI Official Symbol: DIABLO
• NCBI Official Synonym Symbols: SMAC; SMAC3; DFNA64; DIABLO-S
• NCBI Protein Information: diablo homolog, mitochondrial; 0610041G12Rik; mitochondrial Smac protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase
• UniProt Protein Name: Diablo homolog, mitochondrial
• Protein Family: Diablo
• UniProt Gene Name: DIABLO
• UniProt Synonym Gene Names: SMAC; Smac
• UniProt Entry Name: DBLOH_HUMAN

Uniprot Description

DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: internal side of plasma membrane; mitochondrion; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress

Disease: Deafness, Autosomal Dominant 64