Western Blot (WB)
(Western blot analysis of GFP fusion protein using GFP-Tag antibody at different dilution.)
Rabbit anti-All GFP-tag Polyclonal Antibody | anti-GFP-tag antibody
GFP-tag polyclonal antibody
Reactivity
All
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
GFP-tag; Polyclonal Antibody; GFP-tag polyclonal antibody; Green fluorescent protein; anti-GFP-tag antibody
Host
Rabbit
Reactivity
All
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
GFP-tag polyclonal antibody detects over-expressed or recombinant proteins containing the GFP epitope tag.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-GFP-tag antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:5000
Immunogen
Recombinant protein of full length GFP.
Preparation and Storage
Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of GFP fusion protein using GFP-Tag antibody at different dilution.)
Western Blot (WB)
(Western blot analysis of GFP fusion protein using GFP-Tag antibody at different dilution.)
Related Product Information for anti-GFP-tag antibody
Background: Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
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Product Notes
The GFP-tag (Catalog #AAA3000224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GFP-tag polyclonal antibody reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's GFP-tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:5000. Researchers should empirically determine the suitability of the GFP-tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP-tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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