Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DNA primase small subunit (PRIM1) Standard Curve.)
Human DNA Primase small Subunit (PRIM1) ELISA Kit | PRIM1 elisa kit
Human DNA Primase small Subunit (PRIM1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DNA primase small subunit (PRIM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DNA primase small subunit (PRIM1) Standard Curve.)
Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. The DNA polymerase-alpha/primase complex contains 4 subunits: the polymerase-alpha p180 (POLA) and p68 (POLA2) subunits, and the primase p58 (PRIM2A) and p49 (PRIM1) subunits. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis
NCBI and Uniprot Product Information
NCBI Description
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
PRIM1: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. Heterodimer of a small subunit and a large subunit. Belongs to the eukaryotic-type primase small subunit family.
Protein type: Nucleotide Metabolism - pyrimidine; DNA replication; Nucleotide Metabolism - purine; EC 2.7.7.-; Transferase
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: nucleoplasm; membrane
Molecular Function: DNA primase activity; metal ion binding
Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; telomere maintenance via recombination; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; mitotic cell cycle; telomere maintenance; G1/S transition of mitotic cell cycle
Research Articles on PRIM1
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Product Notes
The Human PRIM1 prim1 (Catalog #AAA9714823) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714823 ELISA Kit recognizes Human PRIM1. It is sometimes possible for the material contained within the vial of "DNA Primase small Subunit (PRIM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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