Rabbit anti-Mouse Microtubule Associated Protein Tau Polyclonal Antibody | anti-MAPt antibody

Polyclonal Antibody to Microtubule Associated Protein Tau (MAPt)

Cat. Num. AAA2027171

• Gene Names: Mapt; Tau; Mtapt; AI413597; AW045860
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: Purification||Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Microtubule Associated Protein Tau; Polyclonal Antibody; Polyclonal Antibody to Microtubule Associated Protein Tau (MAPt); anti-MAPt antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Purification||Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.34mg/ml (varies by lot)

• Applicable Applications for anti-MAPt antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Organism Species: Mus musculus (Mouse)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Mouse
• Immunogen: Recombinant MAPT (Ala92~Val400) expressed in E.coli (MBS2031298)
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant MAPt, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Brain Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Testis Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 84370347
• NCBI GeneID: 17762
• NCBI Accession #: NP_001033698.1
• NCBI GenBank Nucleotide #: NM_001038609.2
• UniProt Accession #: P10637; P10638; Q60684; Q60685; Q60686; Q62286; Q91WK4; A2A5Y9
• NCBI Official Full Name: microtubule-associated protein tau isoform a
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: Tau; Mtapt; AI413597; AW045860
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 11 67.79 cM|11 E1

Cellular Component: axolemma; axon; axonal growth cone; axoneme; cell body; cell projection; cell soma; cytoplasm; cytoplasmic ribonucleoprotein granule; cytoplasmic side of plasma membrane; cytoskeleton; cytosol; dendrite; growth cone; main axon; membrane; microtubule; microtubule associated complex; microtubule cytoskeleton; neuron projection; nuclear periphery; nuclear speck; nucleus; plasma membrane; postsynaptic density; somatodendritic compartment; tubulin complex

Molecular Function: apolipoprotein binding; DNA binding; enzyme binding; Hsp90 protein binding; identical protein binding; lipoprotein particle binding; microtubule binding; protein binding; protein complex binding; protein domain specific binding; protein homodimerization activity; protein kinase binding; protein phosphatase 2A binding; SH3 domain binding; tubulin binding

Biological Process: adult walking behavior; amyloid fibril formation; apoptosis; axon cargo transport; axon extension; axonogenesis; induction of apoptosis by oxidative stress; intracellular distribution of mitochondria; learning and/or memory; memory; microtubule cytoskeleton organization; mitochondrion transport along microtubule; negative regulation of gene expression; negative regulation of intracellular transport; negative regulation of kinase activity; negative regulation of mitochondrial fission; negative regulation of mitochondrial membrane potential; neuron migration; positive regulation of axon extension; positive regulation of long term synaptic depression; positive regulation of microtubule polymerization; positive regulation of neuron death; positive regulation of superoxide anion generation; protein homooligomerization; regulation of autophagy; regulation of cellular response to heat; regulation of microtubule polymerization or depolymerization; regulation of response to DNA damage stimulus; response to nutrient; response to organic substance; synapse organization; synaptogenesis