RAB35 recombinant protein

Recombinant Human RAB35, Member RAS Oncogene Family

Cat. Num. AAA144604

• Gene Names: RAB35; RAY; H-ray; RAB1C
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: RAB35; Recombinant Human RAB35; Member RAS Oncogene Family; RAB35 Human; Member RAS Oncogene Family Human Recombinant; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C; RAB1C; RAY; RAB35 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: RAB35 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C
• Sequence Length: 152

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for RAB35 recombinant protein

Description: RAB35 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 221 amino acids (1-201 a.a.) and having a molecular mass of 25.2kDa.RAB35 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Ras-related protein Rab-35 (RAB35) is restricted to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis, which is consistent with a central requirement for Rab35-regulated recycling during cell division.

Product Categories/Family for RAB35 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Member RAS Oncogene Family

NCBI and Uniprot Product Information

• NCBI GI #: 262527293
• NCBI GeneID: 11021
• NCBI Accession #: NP_001161078.1
• NCBI GenBank Nucleotide #: NM_001167606.1
• UniProt Accession #: Q15286; B2R6E0; B4E390
• Molecular Weight: 17,058 Da
• NCBI Official Full Name: ras-related protein Rab-35 isoform 2
• NCBI Official Synonym Full Names: RAB35, member RAS oncogene family
• NCBI Official Symbol: RAB35
• NCBI Official Synonym Symbols: RAY; H-ray; RAB1C
• NCBI Protein Information: ras-related protein Rab-35; GTP-binding protein RAY; ras-related protein rab-1c (GTP-binding protein ray)
• UniProt Protein Name: Ras-related protein Rab-35
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB35
• UniProt Synonym Gene Names: RAB1C; RAY
• UniProt Entry Name: RAB35_HUMAN

Uniprot Description

RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; intercellular bridge; mitochondrion; cell projection membrane; melanosome; plasma membrane; endosome membrane; coated pit; clathrin-coated endocytic vesicle

Molecular Function: GTPase activity; protein binding; phosphatidylinositol-4,5-bisphosphate binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; antigen processing and presentation; ER to Golgi vesicle-mediated transport; protein localization; metabolic process; cytokinesis; plasma membrane to endosome transport; endosome transport; endocytic recycling; Rab protein signal transduction; neurite development