Rabbit anti-Human RAB35 Polyclonal Antibody | anti-RAB35 antibody

RAB35 Antibody

Cat. Num. AAA1492063

• Gene Names: RAB35; RAY; H-ray; RAB1C
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: RAB35; Polyclonal Antibody; RAB35 Antibody; Ras-related protein Rab-35; RAB1C; RAY; GTP-binding protein RAY; Ras-related protein Rab-1C; anti-RAB35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 152

• Applicable Applications for anti-RAB35 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Ras-related protein Rab-35 protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1492063 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells cells using MBS1492063 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-RAB35 antibody

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.

NCBI and Uniprot Product Information

• NCBI GI #: 262527293
• NCBI GeneID: 11021
• NCBI Accession #: NP_001161078.1
• NCBI GenBank Nucleotide #: NM_001167606.1
• UniProt Accession #: Q15286; B2R6E0; B4E390
• Molecular Weight: 17,058 Da
• NCBI Official Full Name: ras-related protein Rab-35 isoform 2
• NCBI Official Synonym Full Names: RAB35, member RAS oncogene family
• NCBI Official Symbol: RAB35
• NCBI Official Synonym Symbols: RAY; H-ray; RAB1C
• NCBI Protein Information: ras-related protein Rab-35
• UniProt Protein Name: Ras-related protein Rab-35
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB35
• UniProt Synonym Gene Names: RAB1C; RAY
• UniProt Entry Name: RAB35_HUMAN

Uniprot Description

RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell projection membrane; clathrin-coated endocytic vesicle; coated pit; endoplasmic reticulum membrane; endosome membrane; Golgi membrane; intercellular bridge; melanosome; mitochondrion; plasma membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding

Biological Process: antigen processing and presentation; cytokinesis; endocytic recycling; endosome transport; ER to Golgi vesicle-mediated transport; neurite development; plasma membrane to endosome transport; protein localization; protein transport; small GTPase mediated signal transduction