Human Pentraxin-3 CLIA Kit | PTX3 clia kit

Pentraxin-3 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510995

• Gene Names: PTX3; TSG-14; TNFAIP5
• Reactivity: Human
• Synonyms: Pentraxin-3; Pentraxin-3 (Human) LumiAb ELISA Kit; Human Pentraxin-3; Pentraxin-related protein PTX3; Pentaxin-related protein PTX3; Tumor necrosis factor alpha-induced protein 5; TNF alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; TSG-14; TNFAIP5; TSG14; PTX3 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human PAI-1 ELISA is capable of recognizing both recombinant and naturally produced Human PAI-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 381

• Assay Type: Quantitative Sandwich
• Sensitivity: 11 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTX3 clia kit

Principle of the Assay: The LumiAb Human PAI-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human PAI-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human PAI-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human PAI-1 or Plasminogen Activator Inhibitor 1, also known as Serpin Peptidase Inhibitor Clade E, is a 402 amino acid cytokine protein that acts as "bait" for tissue plasminogen activator, urokinase and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. PAI-1 is expressed from the SERPINE1 gene located at locus 7q21.3-7q22 on chromosome 7. After initial synthesis, the 23 residue signal sequence is removed, allowing for the proper folding and maturation of the PAI-1 protein. Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D), a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery; affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Moreover, high concentrations of PAI-1 seem to contribute to the development of venous but not arterial occlusions. PAI-1 is typically found in plasma and platelets as well as in endothelial, hepatoma and fibrosarcoma cells. A usual post-translational modification that occurs is the inactivation of PAI-1 by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving Arg369-Met370 peptide bond.

Product Categories/Family for PTX3 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 167900484
• NCBI GeneID: 5806
• NCBI Accession #: NP_002843.2
• NCBI GenBank Nucleotide #: NM_002852.4
• UniProt Accession #: P26022
• NCBI Official Full Name: pentraxin-related protein PTX3
• NCBI Official Synonym Full Names: pentraxin 3
• NCBI Official Symbol: PTX3
• NCBI Official Synonym Symbols: TSG-14; TNFAIP5
• NCBI Protein Information: pentraxin-related protein PTX3
• UniProt Protein Name: Pentraxin-related protein PTX3
• Protein Family: Pentraxin-related protein
• UniProt Gene Name: PTX3
• UniProt Synonym Gene Names: TNFAIP5; TSG14; TNF alpha-induced protein 5; TSG-14
• UniProt Entry Name: PTX3_HUMAN

NCBI Description

This gene encodes a member of the pentraxin protein family. The expression of this protein is induced by inflammatory cytokines in response to inflammatory stimuli in several mesenchymal and epithelial cell types, particularly endothelial cells and mononuclear phagocytes. The protein promotes fibrocyte differentiation and is involved in regulating inflammation and complement activation. It also plays a role in angiogenesis and tissue remodeling. The protein serves as a biomarker for several inflammatory conditions. [provided by RefSeq, Jun 2016]

Uniprot Description

PTX3: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q25

Cellular Component: extracellular space; extracellular region

Molecular Function: complement component C1q binding; zymosan binding; virion binding

Biological Process: positive regulation of nitric oxide biosynthetic process; negative regulation of virion penetration into host cell; positive regulation of phagocytosis; innate immune response; response to yeast; opsonization; inflammatory response