Human PTX3/Pentraxin 3 ELISA Kit | PTX3 elisa kit

Human PTX3/Pentraxin 3 Quick ELISA Kit

Cat. Num. AAA1753937

• Gene Names: PTX3; TSG-14; TNFAIP5
• Reactivity: Human
• Synonyms: PTX3/Pentraxin 3; Human PTX3/Pentraxin 3 Quick ELISA Kit; PTX3; TNFAIP5; TSG14; Pentraxin-related protein PTX3; Pentaxin-related protein PTX3; Tumor necrosis factor alpha-induced protein 5; TNF alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; TSG-14; pentraxin 3; PTX3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human PTX3

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum, plasma (EDTA) and saliva
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000; O.D.|0.033|0.154|0.281|0.498|0.937|1.559|2.039|2.195
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|687|1807|9209|; Standard deviation|37.09|110.22|635.42|; CV(%)|5.4%|6.1%|6.9|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|814|1923|9534; Standard deviation|56.16|138.45|762.72; CV(%)|6.9%|7.2%|8.0%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 687|618|703|627|658|36.19|5.5%; Sample 2: 1807|1579|1636|1784|1701|81.64|4.8%; Sample 3: 9209|8917|8805|8754|8921|330.07|3.7%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E18-S381
• Anticoagulant: EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human PTX3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; ; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human PTX3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay; specially designed to measure Human PTX3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for PTX3. The detection antibody is a HRP linked goat polyclonal antibody specific for PTX3. The kit contains recombinant Human PTX3 with immunogen: Expression system for standard: NS0; Immunogen sequence: E18-S381. The kit is analytically validated with ready to use reagents.; ; To measure Human PTX3, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PTX3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PTX3 in the sample.
• Assay Procedure Step 1: 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for PTX3 elisa kit

PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25. 32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Product Categories/Family for PTX3 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 167900484
• NCBI GeneID: 5806
• NCBI Accession #: NP_002843.2
• NCBI GenBank Nucleotide #: NM_002852.3
• UniProt Accession #: P26022; Q38M82; B2R6T6
• Molecular Weight: 41,976 Da
• NCBI Official Full Name: pentraxin-related protein PTX3
• NCBI Official Synonym Full Names: pentraxin 3, long
• NCBI Official Symbol: PTX3
• NCBI Official Synonym Symbols: TSG-14; TNFAIP5
• NCBI Protein Information: pentraxin-related protein PTX3; pentraxin-3; TNF alpha-induced protein 5; pentaxin-related protein PTX3; tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; tumor necrosis factor-inducible protein TSG-14; tumor n
• UniProt Protein Name: Pentraxin-related protein PTX3
• Protein Family: Pentraxin-related protein
• UniProt Gene Name: PTX3
• UniProt Synonym Gene Names: TNFAIP5; TSG14; TNF alpha-induced protein 5; TSG-14
• UniProt Entry Name: PTX3_HUMAN

Uniprot Description

PTX3: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q25

Cellular Component: extracellular space; extracellular region

Molecular Function: complement component C1q binding; zymosan binding; virion binding

Biological Process: positive regulation of nitric oxide biosynthetic process; negative regulation of virion penetration into host cell; positive regulation of phagocytosis; innate immune response; response to yeast; opsonization; inflammatory response