PTK6 recombinant protein

Recombinant PTK6 protein

Cat. Num. AAA389383

• Gene Names: PTK6; BRK
• Synonyms: PTK6; Recombinant PTK6 protein; Tyrosine-protein kinase BRK; PTK6 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant PTK6 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PTK6 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 53 kDa Purity: >95%)

Related Product Information for PTK6 recombinant protein

Short Description: Recombinant PTK6 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_005966.1) with a N-terminal DYKDDDDK Tag. The molecular weight of the protein is 53.1 kDa.

Background: PTK6 is a non-receptor tyrosine-protein kinase which implicated in the regulation of a variety of signaling pathways and control the differentiation and maintenance of normal epithelia, as well as tumor growth. Its function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. It also associates with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. PTK6 also contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/ p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS.

Product Categories/Family for PTK6 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 5174647
• NCBI GeneID: 5753
• NCBI Accession #: NP_005966.1
• NCBI GenBank Nucleotide #: NM_005975.3
• Molecular Weight: 14,465 Da
• NCBI Official Full Name: protein-tyrosine kinase 6 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine kinase 6
• NCBI Official Symbol: PTK6
• NCBI Official Synonym Symbols: BRK
• NCBI Protein Information: protein-tyrosine kinase 6; breast tumor kinase; protein-tyrosine kinase BRK; tyrosine-protein kinase BRK; PTK6 protein tyrosine kinase 6
• Protein Family: Protein-tyrosine kinase

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]