SDS-Page
(SDS-PAGE of native human 23kDa p23 protein (SPR-303).)
p23 Recombinant Protein | PTGES3 recombinant protein
Recombinant Human p23
Gene Names
PTGES3; P23; TEBP; cPGES
Reactivity
Human
Applications
Western Blot
Synonyms
p23; Recombinant Human p23; Sid 3177; Co chaperone p23; cPGES; Hsp90 co chaperone; cytosolic prostaglandin E2 synthase; PTGES3; TEBP; PTGES3 recombinant protein
Host
Human
Reactivity
Human
Form/Format
Multi-step Chromatographically Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
160
Applicable Applications for PTGES3 recombinant protein
Western Blot (WB), functional/activity studies
Certificate of Analysis
This product has been certified >90% pure using SDS PAGE analysis. 4uM SPR-303, when added to 2uM SPR-300 (Aha1)-activated Hsp90 (2uM; His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
Conjugate Tag
Native sequence
Expression System
E Coli
Storage Buffer
20mM HEPES buffer pH7.2, 80mM NaCl, 10% glyercol
Preparation and Storage
-20 degree C
SDS-Page
(SDS-PAGE of native human 23kDa p23 protein (SPR-303).)
SDS-Page
(SDS-PAGE of native human 23kDa p23 protein (SPR-303).)
Related Product Information for PTGES3 recombinant protein
Background Info: Molecular weight of approximately 23kDa
Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the Hsp90-progesterone receptor complex along with hsp70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate-mediated hsp90 binding of client proteins (8). Since many hsp90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). Hsp90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the Hsp90-progesterone receptor complex along with hsp70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate-mediated hsp90 binding of client proteins (8). Since many hsp90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). Hsp90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
Product Categories/Family for PTGES3 recombinant protein
References
1. Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23kD
NCBI Official Full Name
prostaglandin E synthase 3 isoform a
NCBI Official Synonym Full Names
prostaglandin E synthase 3 (cytosolic)
NCBI Official Symbol
PTGES3
NCBI Official Synonym Symbols
P23; TEBP; cPGES
NCBI Protein Information
prostaglandin E synthase 3; Hsp90 co-chaperone; telomerase-binding protein p23; progesterone receptor complex p23; cytosolic prostaglandin E synthase; cytosolic prostaglandin E2 synthase; unactive progesterone receptor, 23 kD
UniProt Protein Name
Prostaglandin E synthase 3
Protein Family
UniProt Gene Name
PTGES3
UniProt Synonym Gene Names
P23; TEBP; cPGES
UniProt Entry Name
TEBP_HUMAN
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Product Notes
The PTGES3 ptges3 (Catalog #AAA806950) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human p23 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p23 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), functional/activity studies. Researchers should empirically determine the suitability of the PTGES3 ptges3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p23, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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