SDS-Page
(SDS-PAGE of native human 38kDa Aha1 protein (SPR-300).)
Aha1 Recombinant Protein | AHSA1 recombinant protein
Recombinant Human AHA1
Gene Names
AHSA1; p38; AHA1; C14orf3
Reactivity
Human
Applications
Western Blot
Synonyms
Aha1; Recombinant Human AHA1; SHSA1; HspC322; p38; AHSA1 recombinant protein
Host
Human
Reactivity
Human
Form/Format
Multi-step Chromatographically Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
338
Applicable Applications for AHSA1 recombinant protein
Western Blot (WB) control, functional/activity studies
Certificate of Analysis
This product has been certified >90% pure using SDS PAGE analysis. 2uM SPR-300 generated a 9-fold ATPase activation of 2uM Hsp90 (His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
Conjugate Tag
Native sequence
Expression System
E Coli
Storage Buffer
20mM HEPES buffer pH7.2, 80mM NaCl, 10% glyercol
Preparation and Storage
-20 degree C
SDS-Page
(SDS-PAGE of native human 38kDa Aha1 protein (SPR-300).)
SDS-Page
(SDS-PAGE of native human 38kDa Aha1 protein (SPR-300).)
Related Product Information for AHSA1 recombinant protein
Background Info: Molecular weight of approximately 38kDa
Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Product Categories/Family for AHSA1 recombinant protein
References
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38kD
NCBI Official Full Name
activator of 90 kDa heat shock protein ATPase homolog 1
NCBI Official Synonym Full Names
AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
NCBI Official Symbol
AHSA1
NCBI Official Synonym Symbols
p38; AHA1; C14orf3
NCBI Protein Information
activator of 90 kDa heat shock protein ATPase homolog 1
UniProt Protein Name
Activator of 90 kDa heat shock protein ATPase homolog 1
Protein Family
UniProt Gene Name
AHSA1
UniProt Synonym Gene Names
C14orf3; HSPC322; AHA1
UniProt Entry Name
AHSA1_HUMAN
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Product Notes
The AHSA1 ahsa1 (Catalog #AAA806944) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human AHA1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) control, functional/activity studies. Researchers should empirically determine the suitability of the AHSA1 ahsa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aha1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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