Proteasome Subunit Alpha Type 1 Recombinant Protein | PSMa1 recombinant protein

Recombinant Proteasome Subunit Alpha Type 1 (PSMa1)

Cat. Num. AAA2028950

• Gene Names: PSMA1; NU; HC2; PROS30; HEL-S-275
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Proteasome Subunit Alpha Type 1; Recombinant Proteasome Subunit Alpha Type 1 (PSMa1); PSMa1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; QGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALKRAQSELAAHQ KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFDRPLPVSRLVS LIGSKTQIPT QRYGRRPYGV GLLIAGYDDM GPHIFQTCPSANYFDCRAMS IGARSQSART YLERHMSEFM ECNLNELVKH GLRALRETLPAEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLEGLEERP QRKAQPAQPADEPAEKADEP MEH
• Sequence Length: 269

• Applicable Applications for PSMa1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.4kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln16~His263
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 23110935
• NCBI GeneID: 5682
• NCBI Accession #: NP_683877.1
• NCBI GenBank Nucleotide #: NM_148976.2
• Molecular Weight: 30,239 Da
• NCBI Official Full Name: proteasome subunit alpha type-1 isoform 1
• NCBI Official Synonym Full Names: proteasome subunit alpha 1
• NCBI Official Symbol: PSMA1
• NCBI Official Synonym Symbols: NU; HC2; PROS30; HEL-S-275
• NCBI Protein Information: proteasome subunit alpha type-1
• UniProt Protein Name: Proteasome subunit alpha type-1
• Protein Family: Proteasome
• UniProt Gene Name: PSMA1
• UniProt Synonym Gene Names: HC2; NU; PROS30; PSC2; PROS-30
• UniProt Entry Name: PSA1_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

PSMA1: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome. Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; polysome; centrosome; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; protein binding; RNA binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis