Human P selectin glycoprotein ligand 1 ELISA Kit | PSGL 1 elisa kit

Human P selectin glycoprotein ligand 1 ELISA Kit

Cat. Num. AAA730025

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Human
• Synonyms: P selectin glycoprotein ligand 1; Human P selectin glycoprotein ligand 1 ELISA Kit; PSGL 1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSGL1. No significant cross-reactivity or interference between PSGL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSGL1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSGL 1 elisa kit

Intended Uses: This PSGL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PSGL1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: PSGL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PSGL1 antibody and an PSGL1-HRP conjugate. The assay sample and buffer are incubated together with PSGL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSGL1 concentration since PSGL1 from samples and PSGL1-HRP conjugate compete for the anti-PSGL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PSGL1 from the sample, fewer sites are left to bind PSGL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSGL1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 331284236
• NCBI GeneID: 6404
• NCBI Accession #: NP_002997.2
• NCBI GenBank Nucleotide #: NM_003006.4
• Molecular Weight: 44,648 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 2
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: blood coagulation; leukocyte tethering or rolling; cell adhesion; leukocyte migration; leukocyte adhesive activation