Western Blot (WB)
((0. 03ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat cardiac troponin T Polyclonal Antibody | anti-TNNT2 antibody
Goat anti-cardiac troponin T Antibody
Western Blot: Approx 40kDa band observed in Human, fetal Mouse and adult Rat and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2 and 35.7kDa according to Rat NP_036808.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde et al, Cardiovasc Res.2010 Jun 1;86(3): 452-60. PMID: 20083571). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.
Protein type: Motor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: cytosol; sarcomere; striated muscle thin filament; troponin complex
Molecular Function: actin binding; ATPase activity; protein binding; protein binding, bridging; tropomyosin binding; troponin C binding; troponin I binding
Biological Process: actin crosslink formation; cardiac muscle contraction; muscle filament sliding; negative regulation of ATPase activity; positive regulation of ATPase activity; protein heterooligomerization; regulation of heart contraction; regulation of muscle filament sliding speed; response to calcium ion; ventricular cardiac muscle morphogenesis
Disease: Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2; Cardiomyopathy, Familial Restrictive, 3
Research Articles on TNNT2
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Product Notes
The TNNT2 tnnt2 (Catalog #AAA423139) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-cardiac troponin T Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Cat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's cardiac troponin T can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 40kDa band observed in Human, fetal Mouse and adult Rat and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2 and 35.7kDa according to Rat NP_036808.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde et al, Cardiovasc Res.2010 Jun 1;86(3): 452-60. PMID: 20083571). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the TNNT2 tnnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RNRINDNQKV SKT. It is sometimes possible for the material contained within the vial of "cardiac troponin T, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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