Typical Testing Data/Standard Curve (for reference only)
Goat Presenilin 2 ELISA Kit | PS2 elisa kit
Goat Presenilin 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PS2 antibody and an PS2-HRP conjugate. The assay sample and buffer are incubated together with PS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PS2 concentration since PS2 from samples and PS2-HRP conjugate compete for the anti-PS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PS2 from the sample, fewer sites are left to bind PS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PS2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
PSEN2: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). An integral membrane protein of the Golgi and endoplasmic reticulum. Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. Two alternative splice isoforms have been identified.
Protein type: Membrane protein, multi-pass; Protease; EC 3.4.23.-; Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q42.13
Cellular Component: Golgi apparatus; centrosome; cell surface; lysosomal membrane; integral to plasma membrane; nuclear inner membrane; cytosol; lipid raft; kinetochore; ciliary rootlet; membrane; cell soma; perinuclear region of cytoplasm; mitochondrial inner membrane; apical plasma membrane; dendritic shaft; neuromuscular junction; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cell cortex; Z disc; Golgi membrane; growth cone; axon; plasma membrane
Molecular Function: protein binding; endopeptidase activity; aspartic-type endopeptidase activity
Biological Process: positive regulation of catalytic activity; axon guidance; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; protein maturation; regulation of synaptic plasticity; cell fate specification; positive regulation of coagulation; T cell receptor signaling pathway; T cell activation during immune response; amyloid precursor protein catabolic process; protein transport; negative regulation of protein amino acid phosphorylation; hair follicle development; calcium ion transport; beta-amyloid metabolic process; forebrain development; brain morphogenesis; ephrin receptor signaling pathway; negative regulation of protein binding; dorsoventral neural tube patterning; cardiac muscle contraction; embryonic limb morphogenesis; Notch signaling pathway; somitogenesis; membrane protein intracellular domain proteolysis; thymus development; regulation of epidermal growth factor receptor activity; membrane protein ectodomain proteolysis; Notch receptor processing; memory; endoplasmic reticulum calcium ion homeostasis; response to hypoxia; myeloid leukocyte differentiation; hemopoietic progenitor cell differentiation; protein processing; alveolus development; negative regulation of protein complex assembly; negative regulation of apoptosis
Disease: Alzheimer Disease 4; Cardiomyopathy, Dilated, 1v
Research Articles on PS2
Similar Products
Product Notes
The Goat PS2 psen2 (Catalog #AAA749710) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA749710 ELISA Kit recognizes Goat PS2. It is sometimes possible for the material contained within the vial of "Presenilin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
