Immunoprecipitation (IP)
(C19ORF10 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate1 uL anti-C19ORF10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-C19ORF10 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 19 kDa)
Rabbit anti-Human SF20 Polyclonal Antibody | anti-MYDGF antibody
Anti-SF20 Antibody, Rabbit Polyclonal
IP: 0.1-0.5uL/mg of lysate
Immunoprecipitation (IP)
(C19ORF10 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate1 uL anti-C19ORF10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-C19ORF10 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 19 kDa)
Immunoprecipitation (IP)
(C19ORF10 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate1 uL anti-C19ORF10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-C19ORF10 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 19 kDa)
Western Blot (WB)
(Anti-C19ORF10 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:19 kDaObserved band size:16 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-C19ORF10 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:19 kDaObserved band size:16 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Uniprot Description
MYDGF: was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular space; endoplasmic reticulum lumen; ER-Golgi intermediate compartment
Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; positive regulation of MAPKKK cascade; unfolded protein response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis
Research Articles on MYDGF
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Product Notes
The MYDGF c19orf10 (Catalog #AAA8106948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SF20 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SF20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 0.1-0.5uL/mg of lysate. Researchers should empirically determine the suitability of the MYDGF c19orf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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