Protease, Serine 12 (PRSS12) Recombinant Protein | PRSS12 recombinant protein

Recombinant Protease, Serine 12 (PRSS12)

Cat. Num. AAA2018548

• Gene Names: Prss12; Bssp-3; motopsin
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protease; Serine 12 (PRSS12); Recombinant Protease; PRSS12 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GYCDCGQG PALPVIRLVG GNSGHEGRVE LYHAGQWGTI CDDQWDNADA DVICRQLGLS GIAKAWHQAH FGEGSGPILL DEVRCTGNEL SIEQCPKSSW GEHNCGHKED AGVSCVPLTD GVIRLAGGKS THEGRLEVYY KGQWGTVCDD GWTEMNTYVA CRLLGFKYGK QSSVNHFDGS NRPIWLDDVS CSGKEVSFIQ CSRRQWGRHD CSHREDVGLT CYPDSDGHRL SPGFPIRLVD GENKKEGRV
• Sequence Length: 761

• Applicable Applications for PRSS12 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly153~Val399 (Accession # O08762) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6679485
• NCBI GeneID: 19142
• NCBI Accession #: NP_032965.1
• NCBI GenBank Nucleotide #: NM_008939.2
• UniProt Accession #: O08762
• Molecular Weight: 30.9kDa
• NCBI Official Full Name: neurotrypsin
• NCBI Official Synonym Full Names: protease, serine 12 neurotrypsin (motopsin)
• NCBI Official Symbol: Prss12
• NCBI Official Synonym Symbols: Bssp-3; motopsin
• NCBI Protein Information: neurotrypsin; serine protease 12; brain-specific serine protease 3; protease, serine, 12 neurotrypsin (motopsin)
• UniProt Protein Name: Neurotrypsin
• Protein Family: Neurotrypsin
• UniProt Gene Name: Prss12
• UniProt Synonym Gene Names: Bssp3; BSSP-3
• UniProt Entry Name: NETR_MOUSE

Uniprot Description

PRSS12: Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations. Defects in PRSS12 are the cause of mental retardation autosomal recessive type 1 (MRT1). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Vesicle; EC 3.4.21.-; Protease; Secreted

Cellular Component: membrane; axon; dendrite; plasma membrane; extracellular region; terminal button; synapse; cytoplasmic vesicle

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity; scavenger receptor activity

Biological Process: exocytosis; zymogen activation; proteolysis