Rabbit anti-Human Matrix Metalloproteinase 1 Polyclonal Antibody | anti-MMP-1 antibody

Matrix Metalloproteinase 1 (MMP-1, Interstitial Collagenase 1) (BSA & Azide Free) (APC)

Cat. Num. AAA6486948

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Matrix Metalloproteinase 1; Polyclonal Antibody; Matrix Metalloproteinase 1 (MMP-1; Interstitial Collagenase 1) (BSA & Azide Free) (APC); anti-MMP-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Matrix Metalloproteinase 1 (MMP-1, Interstitial Collagenase 1). Species Crossreactivity: human. Does not react with bovine.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MMP-1 antibody: Immunohistochemistry (IHC) Frozen/Paraffin
• Application Notes: IHC-F/P: (Use Ab at 10-20ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.]; Applications are based on unconjugated antibody.
• Immunogen: A synthetic peptide from the middle region of the human MMP-1
• Conjugate: APC
• Positive Control: Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Placenta. Bladder, breast, ovarian carcinoma.
• Epitope: Middle region of MMP-1
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot)

Product Categories/Family for anti-MMP-1 antibody

Antibodies; Matrix Metalloproteinases

References

1. Birkedal-Hansen B, et. al. Biochemistry, 1988;27:6751-6758. 2. Windsor LJ, et. al. Biochemistry, 1991;30:641-647. Additional: 1. Heissenberg MC, Gorogh T, Lippert BM, Werner JA: Metalloproteinases and their inhibitors in squamous cell carcinoma of the hypopharynx: indicators of individual tumor aggressiveness. Otolaryngol Pol 1998;52(5):521-526. 2. Kataoka H, DeCastro R, Zucker S, Biswas C: Tumor cell-derived collagenase-stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72-kDa gelatinase. Cancer Res 1993 Jul 1;53(13):3154-3158. 3. Sang QX: Complex role of matrix metalloproteinases in angiogenesis. Cell Res 1998;8(3):171-177. 4. Takiguchi T, Kobayashi M, Suzuki R, Yamaguchi A, Isatsu K, Nishihara T, Nagumo M, Hasegawa K: Recombinant human bone morphogenetic protein-2 stimulates osteoblast differentiation and suppresses matrix metalloproteinase-1 production in human bone cells isolated from mandibulae. J Periodontal Res 1998 Nov;33(8):476-485. 5. Lim M, Martinez T, Jablons D, Cameron R, Guo H, Toole B, Li JD, Basbaum C: Tumor-derived EMMPRIN (extracellular matrix metalloproteinase inducer) stimulates collagenase transcription through MAPK p38. FEBS Lett 1998 Dec 11;441(1):88-92. 6. Benbow U, Rutter JL, Lowrey CH, Brinckerhoff CE: Transcriptional repression of the human collagenase-1 (MMP-1) gene in MDA231 breast cancer cells by all-trans-retinoic acid requires distal regions of the promoter. Br J Cancer 1999 Feb;79(2):221-228. 7. Levin JI, DiJoseph JF, Killar LM, Sharr MA, Skotnicki JS, Patel DV, Xiao XY, Shi L, Navre M, Campbell DA: The asymmetric synthesis and in vitro characterization of succinyl mercaptoalcohol and mercaptoketone inhibitors of matrix metalloproteinases. Bioorg Med Chem Lett 1998 May 19;8(10):1163-1168. 8. Andersen K, Maelandsmo GM, Hovig E, Fodstad O, Loennechen T, Winberg JO: Interleukin-1 alpha and basic fibroblast growth factor induction of matrix metalloproteinases and their inhibitors in osteosarcoma cells is modulated by the metastasis associated protein CAPL. Anticancer Res 1998 Sep-Oct;18(5A):3299-3303. 9. Zygmunt M, Hahn D, Kiesenbauer N, Munstedt K, Lang U: Invasion of cytotrophoblastic (JEG-3) cells is Up-regulated by interleukin-15 In Vitro. Am J Reprod Immunol 1998 Nov;40(5):326-331.