Human Vitamin K-dependent protein Z ELISA Kit | PROZ elisa kit

Human Vitamin K-dependent protein Z ELISA Kit

Cat. Num. AAA765710

• Gene Names: PROZ; PZ
• Reactivity: Human
• Synonyms: Vitamin K-dependent protein Z; Human Vitamin K-dependent protein Z ELISA Kit; PROZ (Vitamin K-dependent protein Z)/protein Z; PROZ elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pros1. No significant cross-reactivity or interference between Pros1 and analogues was observed.
• Sequence Length: 400

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/ml
• Sensitivity: <0.375ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pros1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pros1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PROZ elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Pros1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Pros1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Pros1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Pros1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506121
• NCBI GeneID: 8858
• NCBI Accession #: NP_003882.1
• NCBI GenBank Nucleotide #: NM_003891.2
• Molecular Weight: 47,053 Da
• NCBI Official Full Name: vitamin K-dependent protein Z isoform 2
• NCBI Official Synonym Full Names: protein Z, vitamin K-dependent plasma glycoprotein
• NCBI Official Symbol: PROZ
• NCBI Official Synonym Symbols: PZ
• NCBI Protein Information: vitamin K-dependent protein Z
• UniProt Protein Name: Vitamin K-dependent protein Z
• Protein Family: Vitamin K-dependent protein
• UniProt Gene Name: PROZ
• UniProt Entry Name: PROZ_HUMAN

NCBI Description

This gene encodes a liver vitamin K-dependent glycoprotein that is synthesized in the liver and secreted into the plasma. The encoded protein plays a role in regulating blood coagulation by complexing with protein Z-dependent protease inhibitor to directly inhibit activated factor X at the phospholipid surface. Deficiencies in this protein are associated with an increased risk of ischemic arterial diseases and fetal loss. Mutations in this gene are the cause of protein Z deficiency. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

PROZ: Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; Golgi lumen

Molecular Function: calcium ion binding; serine-type endopeptidase activity

Biological Process: blood coagulation; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; peptidyl-glutamic acid carboxylation; post-translational protein modification; signal peptide processing

Disease: Protein Z Deficiency