Rabbit Protein Z ELISA Kit | Protein Z elisa kit

Rabbit Protein Z ELISA Kit

Cat. Num. AAA730566

• Gene Names: PROZ; PZ
• Reactivity: Rabbit
• Synonyms: Protein Z; Rabbit Protein Z ELISA Kit; Rabbit Protein Z (Protein Z) ELISA Kit; Protein Z (Rabbit); Protein Z elisa kit

• Reactivity: Rabbit

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Protein Z elisa kit

Intended Uses: This Protein Z ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Protein Z in the sample, this Protein Z ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Protein Z concentration. The concentration of Protein Z in the samples is then determined by comparing the O.D. of the samples to the standard curve.f

Principle of the Assay: This Protein Z enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Protein Z. Standards or samples are then added to the microtiter plate wells and Protein Z if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Protein Z present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Protein Z are added to each well to "sandwich" the Protein Z immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain Protein Z and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for Protein Z elisa kit

Rabbit ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 190466
• NCBI GeneID: 8858
• UniProt Accession #: P22891; Q15213; Q5JVF5; Q5JVF6; A6NMB4
• Molecular Weight: 44,744 Da
• NCBI Official Full Name: protein Z
• NCBI Official Synonym Full Names: protein Z, vitamin K-dependent plasma glycoprotein
• NCBI Official Symbol: PROZ
• NCBI Official Synonym Symbols: PZ
• NCBI Protein Information: vitamin K-dependent protein Z; OTTHUMP00000018738; OTTHUMP00000018739
• UniProt Protein Name: Vitamin K-dependent protein Z
• Protein Family: Protein
• UniProt Gene Name: PROZ
• UniProt Entry Name: PROZ_HUMAN

NCBI Description

This gene encodes a liver vitamin K-dependent glycoprotein that is synthesized in the liver and secreted into the plasma. The encoded protein plays a role in regulating blood coagulation by complexing with protein Z-dependent protease inhibitor to directly inhibit activated factor X at the phospholipid surface. Deficiencies in this protein are associated with an increased risk of ischemic arterial diseases and fetal loss. Mutations in this gene are the cause of protein Z deficiency. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

PROZ: Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; Golgi lumen

Molecular Function: serine-type endopeptidase activity; calcium ion binding

Biological Process: cellular protein metabolic process; post-translational protein modification; proteolysis; blood coagulation; peptidyl-glutamic acid carboxylation

Disease: Protein Z Deficiency