CXCL7 Recombinant Protein | PPBP recombinant protein

CXCL7 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123346

• Gene Names: Ppbp; Cxcl7; Nap-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CXCL7; CXCL7 Protein; Rat; Recombinant (His Tag); Rat NAP-2/PPBP/CXCL7 Protein (His Tag); pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); PPBP recombinant protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys38-Tyr111

• Species: Rat
• Predicted N Terminal: Lys 38
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat Cxcl7 (NP_714943.1)(Lys38-Tyr111) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PPBP recombinant protein

Background: Pro-platelet basic protein (PPBP) is also known as Chemokine (C-X-C motif) ligand 7 (CXCL7) and nucleosome assembly protein (Nap-2). Nap-2/PPBP/CXCL7 is released in large amounts from platelets following their activation and is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. Nap-2/PPBP/CXCL7 has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Nap-2 is a ligand for CXCR1 and CXCR2, and Nap-2, Nap-2 (73), Nap-2 (74), Nap-2 (1-66), and most potent Nap-2 (1-63) are chemoattractants and activators for neutrophils.

References

Walz A, et al. (1989) Effects of the neutrophil-activating peptide NAP-2, platelet basic protein, connective tissue-activating peptide III and platelet factor 4 on human neutrophils. J Exp Med. 170(5):1745-50.
Walz A, et al. (1990) Generation of the neutrophil-activating peptide NAP-2 from platelet basic protein or connective tissue-activating peptide III through monocyte proteases. J Exp Med. 171(2): 449-54.
Loetscher P, et al. (1994) Both interleukin-8 receptors independently mediate chemotaxis. Jurkat cells transfected with IL-8R1 or IL-8R2 migrate in response to IL-8, GRO alpha and NAP-2. FEBS Lett. 341(2-3): 187-92.

NCBI and Uniprot Product Information

• NCBI GI #: 24308468
• NCBI GeneID: 246358
• NCBI Accession #: NP_714943.1
• NCBI GenBank Nucleotide #: NM_153721.1
• UniProt Accession #: Q99ME0
• Molecular Weight: 12,159 Da
• NCBI Official Full Name: platelet basic protein
• NCBI Official Synonym Full Names: pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• NCBI Official Symbol: Ppbp
• NCBI Official Synonym Symbols: Cxcl7; Nap-2
• NCBI Protein Information: platelet basic protein; chemokine (C-X-C motif) ligand 7; neutrophil activating peptide-2
• UniProt Protein Name: CXC chemokine RTCK1
• Protein Family: Platelet basic protein
• UniProt Gene Name: Ppbp
• UniProt Entry Name: Q99ME0_RAT

NCBI Description

has 72.6% amino acid sequence identity with the mouse homolog which displays increased expression in response to thrombopoietin stimulation [RGD, Feb 2006]