Testing Data
WWE Protein
WWE Neg Control Resin
Applications
Western Blot, Immunoblot, Western Blot
Purity
GST-WWE fusion protein, purity >95% by SDSPAGE
Synonyms
WWE; WWE Neg Control Resin; WWE protein
Purity/Purification
GST-WWE fusion protein, purity >95% by SDSPAGE
Form/Format
Each vial contains 0.5mg purified GST-WWE fusion protein bound to 50-75ìL packed volume of glutathione beads in 0.5 mL buffer containing 10 mM sodium phosphate, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, and 0.02% sodium azide.
Applicable Applications for WWE protein
Western Blot (WB), Immunoblot (IB)
Application Notes
Use 20uL (20ug) suspended resin to affinity purify/pull-down poly-ADP-ribose modified proteins in 0.15-1mg cell and tissue extracts. Analyze by Western blotting using protein-specific antibodies to probe the immunoblot. Each 0.5mL vial is sufficient for analysis of ~25 samples.
Notes
Neg Control Resin has been shown to bind to purified highly automodified PARP1 under certain conditions.
Preparation and Storage
Stable for 6 months from date of shipment when stored at 4 degree C. DO NOT FREEZE!
Testing Data
Testing Data
Related Product Information for WWE protein
Background: WWE Affinity Resin and Negative Control Resin are designed for the isolation and study of poly-ADP-ribosylated (PARylated) proteins. Through the use of this highly specific PAR affinity resin, PARylated proteins are isolated from cell or tissue lysates without the use of anti-PAR antibodies. The resin-bound proteins can be eluted from the affinity resin, and analyzed by immunoblotting or other methods. RNF146 (Iduna) is a RING domain E3 ubiquitin ligase that positively regulates Wnt signalling. RNF146 directly interacts with poly(ADP-ribose) through its WWE domain. The WWE domain is a conserved globular domain found in multiple PARPs and E3 ligases. WWE Affinity Resin is a GST fusion protein of WWE PARbinding domain of human RNF146, amino acid residues 100-175, bound to a glutathione resin.
Product Description: WWE Negative Control Resin has a single amino acid substitution R163A, which abolishes PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.
Product Description: WWE Negative Control Resin has a single amino acid substitution R163A, which abolishes PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.
References
H.C. Kang et al. (2011) PNAS 108 14103 Y. Zhang et al. (2011) Nature Cell Biol. 13 623 M.G. Callow et al. (2011) PLoS ONE 6 e22595 S.A. Andrabi et al. (2011) Nature Medicine 17 692 Z.D. Zhou et al. (2011) Cell Adh. Migr. 5 463 Z. Wang et al. (2012) Genes & Dev. 26 235
Similar Products
Product Notes
The WWE (Catalog #AAA376023) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's WWE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoblot (IB). Use 20uL (20ug) suspended resin to affinity purify/pull-down poly-ADP-ribose modified proteins in 0.15-1mg cell and tissue extracts. Analyze by Western blotting using protein-specific antibodies to probe the immunoblot. Each 0.5mL vial is sufficient for analysis of ~25 samples. Researchers should empirically determine the suitability of the WWE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WWE, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
