Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GFRA1 antibody (MBS126347) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit GFRA1 Polyclonal Antibody | anti-GFRA1 antibody
GFRA1
Gene Names
GFRA1; GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
GFRA1; Polyclonal Antibody; GFRA1;GDNFR;GDNFRA;GFR-ALPHA-1;RET1L;RETL1;TRNR1; anti-GFRA1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3
Sequence Length
460
Applicable Applications for anti-GFRA1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IF: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human GFRA1 (NP_665736.1).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GFRA1 antibody (MBS126347) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GFRA1 antibody (MBS126347) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using GFRA1 antibody (MBS126347) at dilution of 1:100. Blue: DAPI for nuclearstaining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using GFRA1 antibody (MBS126347) at dilution of 1:100. Blue: DAPI for nuclearstaining.)
Related Product Information for anti-GFRA1 antibody
This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 51kDa
Calculated MW: 50kDa/51kDa
Calculated MW: 50kDa/51kDa
NCBI Official Full Name
GDNF family receptor alpha-1 isoform b preproprotein
NCBI Official Synonym Full Names
GDNF family receptor alpha 1
NCBI Official Symbol
GFRA1
NCBI Official Synonym Symbols
GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1
NCBI Protein Information
GDNF family receptor alpha-1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha-1; GPI-linked anchor protein; PI-linked cell-surface accessory protein; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha
UniProt Protein Name
GDNF family receptor alpha-1
Protein Family
UniProt Gene Name
GFRA1
UniProt Synonym Gene Names
GDNFRA; RETL1; TRNR1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1
UniProt Entry Name
GFRA1_HUMAN
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Product Notes
The GFRA1 gfra1 (Catalog #AAA126347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GFRA1 reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's GFRA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500 - 1:2000 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the GFRA1 gfra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFRA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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