Mouse anti-Human DR3 Monoclonal Antibody | anti-DR3 antibody

DR3 (Death Receptor 3, DR-3, Apo-3, Apoptosis-inducing Receptor AIR, TRAMP, Death Domain Receptor 3, DDR3, Death Receptor beta, Lymphocyte Associated Receptor of Death, LARD, TR3, Translocating Chain Association Membrane Protein, Tumor Necrosis Factor Rec

Cat. Num. AAA6152211

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DR3; Monoclonal Antibody; DR3 (Death Receptor 3; DR-3; Apo-3; Apoptosis-inducing Receptor AIR; TRAMP; Death Domain Receptor 3; DDR3; Death Receptor beta; Lymphocyte Associated Receptor of Death; LARD; TR3; Translocating Chain Association Membrane Protein; Tumor Necrosis Factor Rec; anti-DR3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4E3
• Specificity: Recognizes human TNFRSF25.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-DR3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa28-124 from TNFRSF25 (NP_003781) with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.78kD).)

Testing Data

(Detection limit for recombinant GST tagged TNFRSF25 is 1ng/ml as a capture antibody.)

Related Product Information for anti-DR3 antibody

TNFRSF25 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins.

Product Categories/Family for anti-DR3 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4507569
• NCBI GeneID: 8718
• NCBI Accession #: NP_003781
• NCBI GenBank Nucleotide #: NM_003790
• UniProt Accession #: Q93038; O00275; O00276; O00277; O00278; O00279; O00280; O14865; O14866; B1ALX2; B1ALX3; B7ZLL7
• Molecular Weight: 46.1kDa (417aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 25 isoform 2
• NCBI Official Synonym Full Names: TNF receptor superfamily member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction