Mouse Prolactin ELISA Kit | PRL elisa kit

Mouse Prolactin PicoKine ELISA Kit

Cat. Num. AAA177252

• Gene Names: Prl; Prl1a1; AV290867
• Reactivity: Mouse
• Synonyms: Prolactin; Mouse Prolactin PicoKine ELISA Kit; prolactin; AV290867; Lactogenic Hormone; Luteotropic Hormone; Mammotropin; OTTMUSP00000000651; PRL; Prl1a1; Prol; Prolactin precursor; RATPRLSD1; RNPROL; RP23-351I6.2; RP3-404K8.1; PRL elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Prl
• Sequence Length: 226

• Samples: Cell culture supernatants, serum and plasma (heparin, citrate)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRL elisa kit

Principle of the Assay: The Picokine™ Mouse Prl Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Prl with a 96-well strip plate that is pre-coated with antibody specific for Prl. The detection antibody is a biotinylated antibody specific for Prl. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Prl with immunogen: Expression system for standard: E.coli; Immunogen sequence: L32-C228. The kit is analytically validated with ready-to-use reagents. To measure Mouse Prl, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Prl in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Prl in the sample.

Background/Introduction: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 130934
• NCBI GeneID: 19109
• UniProt Accession #: P06879; Q544X8
• Molecular Weight: 25,496 Da
• NCBI Official Full Name: Prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: Prl
• NCBI Official Synonym Symbols: Prl1a1; AV290867
• NCBI Protein Information: prolactin
• UniProt Protein Name: Prolactin
• Protein Family: Prolactin
• UniProt Gene Name: Prl
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_MOUSE

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Cytokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular region; secretory granule

Molecular Function: prolactin receptor binding; hormone activity

Biological Process: lactation; regulation of JAK-STAT cascade; mammary gland development; maternal behavior; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; regulation of ossification; positive regulation of epithelial cell proliferation; STAT protein nuclear translocation