Typical Testing Data/Standard Curve (for reference only)
Mouse Prolactin ELISA Kit | PRL elisa kit
Mouse Prolactin PicoKine ELISA Kit
Gene Names
Prl; Prl1a1; AV290867
Reactivity
Mouse
Synonyms
Prolactin; Mouse Prolactin PicoKine ELISA Kit; prolactin; AV290867; Lactogenic Hormone; Luteotropic Hormone; Mammotropin; OTTMUSP00000000651; PRL; Prl1a1; Prol; Prolactin precursor; RATPRLSD1; RNPROL; RP23-351I6.2; RP3-404K8.1; PRL elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Prl
Sequence Length
226
Samples
Cell culture supernatants, serum and plasma (heparin, citrate)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRL elisa kit
Principle of the Assay: The Picokine™ Mouse Prl Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Prl with a 96-well strip plate that is pre-coated with antibody specific for Prl. The detection antibody is a biotinylated antibody specific for Prl. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Prl with immunogen: Expression system for standard: E.coli; Immunogen sequence: L32-C228. The kit is analytically validated with ready-to-use reagents. To measure Mouse Prl, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Prl in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Prl in the sample.
Background/Introduction: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
Background/Introduction: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,496 Da
NCBI Official Full Name
Prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
Prl
NCBI Official Synonym Symbols
Prl1a1; AV290867
NCBI Protein Information
prolactin
UniProt Protein Name
Prolactin
Protein Family
UniProt Gene Name
Prl
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_MOUSE
Uniprot Description
prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.
Protein type: Cytokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: extracellular region; secretory granule
Molecular Function: prolactin receptor binding; hormone activity
Biological Process: lactation; regulation of JAK-STAT cascade; mammary gland development; maternal behavior; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; regulation of ossification; positive regulation of epithelial cell proliferation; STAT protein nuclear translocation
Research Articles on PRL
Similar Products
Product Notes
The Mouse PRL prl (Catalog #AAA177252) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177252 ELISA Kit recognizes Mouse PRL. It is sometimes possible for the material contained within the vial of "Prolactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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