Typical Testing Data/Standard Curve (for reference only)
General Prolactin ELISA Kit | PRL elisa kit
Mouse Prolactin ELISA Kit
Gene Names
Prl; Prl1a1; AV290867
Reactivity
General
Synonyms
Prolactin; Mouse Prolactin ELISA Kit; PRL elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
226
Samples
Mouse plasma
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRL elisa kit
Intended Uses: This mouse prolactin antigen assay is intended for the quantitative determination of prolactin antigen in mouse plasma.
Principle of the Assay: Mouse prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinlyated anti-mouse prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total prolactin present in the samples, is reacted with peroxidase-conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prolactin. Color development is proportional to the concentration of prolactin in the samples.
Background: Mouse prolactin (PRL) is a 197 aa, 23kD peptide hormone [1] that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation [2,3]. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk [4].
Principle of the Assay: Mouse prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinlyated anti-mouse prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total prolactin present in the samples, is reacted with peroxidase-conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prolactin. Color development is proportional to the concentration of prolactin in the samples.
Background: Mouse prolactin (PRL) is a 197 aa, 23kD peptide hormone [1] that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation [2,3]. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk [4].
Product Categories/Family for PRL elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,496 Da
NCBI Official Full Name
Prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
Prl
NCBI Official Synonym Symbols
Prl1a1; AV290867
NCBI Protein Information
prolactin
UniProt Protein Name
Prolactin
Protein Family
UniProt Gene Name
Prl
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_MOUSE
Uniprot Description
prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Cytokine; Secreted
Cellular Component: extracellular region; secretory granule
Molecular Function: prolactin receptor binding; hormone activity
Biological Process: lactation; regulation of JAK-STAT cascade; mammary gland development; maternal behavior; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; regulation of ossification; positive regulation of epithelial cell proliferation; STAT protein nuclear translocation
Research Articles on PRL
Similar Products
Product Notes
The General PRL prl (Catalog #AAA135500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135500 ELISA Kit recognizes General PRL. It is sometimes possible for the material contained within the vial of "Prolactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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