Protein Kinase, DNA Activated, Catalytic Polypeptide Recombinant Protein | PRKDC recombinant protein

Recombinant Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC)

Cat. Num. AAA2033257

• Gene Names: Prkdc; p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Kinase; DNA Activated; Catalytic Polypeptide; Recombinant Protein Kinase; Catalytic Polypeptide (PRKDC); HYRC; HYRC1; DNPK1; p350; DNAPK; XRCC7; DNA-PKcs; PRKDC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: AL RSHFASSHAL LCISHWLLGI GDRHLNNFMV AMETGSVIGI DFGHAFGSAT QFLPVPELMP FRLTRQFVSL MLPMKETGLM CTVMVHALRA FRSCAGLLTD TMEIFVKEPS FDWKSFEQTM LRKGGSWIQE INVTEKNWYP QHKIRYAKRK LAGANPAVIT CDELYLGHEA SSAFRSYTAVARGNRDYNIR AQEPESGLSE ETQVKCLVDQ ATDPNILGRT WEGWEPWM
• Sequence Length: 3096

• Applicable Applications for PRKDC recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 29.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Ala3899~Met4128
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 148664987
• NCBI GeneID: 19090
• Molecular Weight: 96,914 Da
• NCBI Official Full Name: protein kinase, DNA activated, catalytic polypeptide
• NCBI Official Synonym Full Names: protein kinase, DNA activated, catalytic polypeptide
• NCBI Official Symbol: Prkdc
• NCBI Official Synonym Symbols: p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
• NCBI Protein Information: DNA-dependent protein kinase catalytic subunit
• UniProt Protein Name: DNA-dependent protein kinase catalytic subunit
• UniProt Gene Name: Prkdc
• UniProt Synonym Gene Names: Xrcc7; DNA-PK catalytic subunit; DNA-PKcs
• UniProt Entry Name: PRKDC_MOUSE

Uniprot Description

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Kinase, protein; Protein kinase, atypical; Nucleolus; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; DNAPK subfamily

Cellular Component: DNA-dependent protein kinase complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleus; transcription factor complex

Molecular Function: ATP binding; DNA binding; DNA-dependent protein kinase activity; double-stranded DNA binding; enzyme binding; kinase activity; nucleotide binding; phosphotransferase activity, alcohol group as acceptor; protein binding; protein kinase activity; protein serine/threonine kinase activity; transcription factor binding; transferase activity

Biological Process: B cell lineage commitment; brain development; cell proliferation; cellular response to insulin stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; germ cell programmed cell death; heart development; immunoglobulin production; immunoglobulin V(D)J recombination; lymphocyte differentiation; negative regulation of apoptosis; negative regulation of immunoglobulin production; negative regulation of protein amino acid phosphorylation; peptidyl-serine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of developmental growth; positive regulation of fibroblast proliferation; positive regulation of immune system process; positive regulation of transcription from RNA polymerase II promoter; pro-B cell differentiation; protein destabilization; regulation of circadian rhythm; regulation of smooth muscle cell proliferation; response to DNA damage stimulus; response to gamma radiation; response to ionizing radiation; rhythmic process; somitogenesis; spleen development; T cell differentiation in the thymus; T cell lineage commitment; T cell receptor V(D)J recombination; telomere capping; telomere maintenance; thymus development; V(D)J recombination