hnRNP-R Recombinant Protein | HNRNPR recombinant protein

hnRNP-R Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121665

• Gene Names: HNRNPR; HNRPR; hnRNP R; hnRNP-R
• Purity: >90% as determined by SDS-PAGE
• Synonyms: hnRNP-R; hnRNP-R Protein; Human; Recombinant (His & GST Tag); Human HNRNPR/HNRNP-R/HNRNP R Protein (His & GST Tag); HNRNPR Protein; HNRPR Protein; heterogeneous nuclear ribonucleoprotein R; HNRNPR recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM GSH, pH8.5, 3mM DTT, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy
• Sequence: Ala2-Lys636

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human HNRNPR (O43390-2)(Ala2-Lys636) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HNRNPR recombinant protein

Background: HNRNP R, also known as HNRNPR, is an RNA binding protein. HNRNP R gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). HnRNPs complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRNP R has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif.

References

Rossoll, et al. (2002) Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons. Hum Mol Genet. 11 (1):93-105. Mourelatos, Z, et al. (2001) SMN interacts with a novel family of hnRNP and spliceosomal proteins. EMBO J. 20(19):5443-52. Hassfeld W, et al. (1998) Molecular definition of heterogeneous nuclear ribonucleoprotein R (hnRNP R) using autoimmune antibody: immunological relationship with hnRNP P. Nucleic Acids Res. 26(2):439-45.

NCBI and Uniprot Product Information

• NCBI GI #: 156151392
• NCBI GeneID: 10236
• NCBI Accession #: NP_001095867.1
• NCBI GenBank Nucleotide #: NM_001102397.1
• UniProt Accession #: O43390; Q2L7G6; Q5TEH1; Q9BV64
• Molecular Weight: 70,943 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein R isoform 4
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein R
• NCBI Official Symbol: HNRNPR
• NCBI Official Synonym Symbols: HNRPR; hnRNP R; hnRNP-R
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein R
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein R
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPR
• UniProt Synonym Gene Names: HNRPR; hnRNP R
• UniProt Entry Name: HNRPR_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.

Subunit structure: Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with GTPBP1. Ref.7 Ref.8 Ref.12

Subcellular location: Nucleus › nucleoplasm. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.8

Sequence similarities: Contains 3 RRM (RNA recognition motif) domains.