Mouse Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit | PRKDC elisa kit

Mouse Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit

Cat. Num. AAA2019433

• Gene Names: Prkdc; p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
• Reactivity: Mouse
• Synonyms: Protein Kinase; DNA Activated; Catalytic Polypeptide (PRKDC); Mouse Protein Kinase; Catalytic Polypeptide (PRKDC) ELISA Kit; HYRC; HYRC1; DNPK1; p350; DNAPK; XRCC7; DNA-PKcs; PRKDC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC).; No significant cross-reactivity or interference between Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) and analogues was observed.
• Sequence Length: 3096

• Assay Type: Double-antibody Sandwich
• Samples: Saliva, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKDC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PRKDC elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 148664987
• NCBI GeneID: 19090
• UniProt Accession #: P97313; O88187; P97928; Q307W9; Q3V2W8; Q8C2A7; Q9Z341; E9QN15
• Molecular Weight: 96,914 Da
• NCBI Official Full Name: protein kinase, DNA activated, catalytic polypeptide
• NCBI Official Synonym Full Names: protein kinase, DNA activated, catalytic polypeptide
• NCBI Official Symbol: Prkdc
• NCBI Official Synonym Symbols: p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
• NCBI Protein Information: DNA-dependent protein kinase catalytic subunit
• UniProt Protein Name: DNA-dependent protein kinase catalytic subunit
• UniProt Gene Name: Prkdc
• UniProt Synonym Gene Names: Xrcc7; DNA-PK catalytic subunit; DNA-PKcs
• UniProt Entry Name: PRKDC_MOUSE

Uniprot Description

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 2.7.11.1; Kinase, protein; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Nucleolus; ATYPICAL group; PIKK family; DNAPK subfamily

Cellular Component: nucleoplasm; transcription factor complex; membrane; intracellular; DNA-dependent protein kinase complex; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; enzyme binding; DNA binding; transferase activity, transferring phosphorus-containing groups; double-stranded DNA binding; nucleotide binding; DNA-dependent protein kinase activity; kinase activity; phosphotransferase activity, alcohol group as acceptor; transcription factor binding; ATP binding

Biological Process: positive regulation of immune system process; positive regulation of apoptosis; heart development; germ cell programmed cell death; T cell differentiation in the thymus; rhythmic process; protein amino acid phosphorylation; double-strand break repair via nonhomologous end joining; positive regulation of fibroblast proliferation; lymphocyte differentiation; negative regulation of protein amino acid phosphorylation; double-strand break repair; response to gamma radiation; immunoglobulin production; telomere maintenance; spleen development; pro-B cell differentiation; somitogenesis; positive regulation of developmental growth; V(D)J recombination; immunoglobulin V(D)J recombination; thymus development; protein destabilization; B cell lineage commitment; DNA repair; regulation of circadian rhythm; double-strand break repair via homologous recombination; T cell lineage commitment; peptidyl-serine phosphorylation; cellular response to insulin stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; T cell receptor V(D)J recombination; positive regulation of transcription from RNA polymerase II promoter; brain development; response to ionizing radiation; phosphorylation; response to DNA damage stimulus