Mouse Mitogen-activated protein kinase 15 ELISA Kit | Mapk15 elisa kit

Mouse Mitogen-activated protein kinase 15 ELISA Kit

Cat. Num. AAA761513

• Gene Names: MAPK15; ERK7; ERK8
• Reactivity: Mouse
• Synonyms: Mitogen-activated protein kinase 15; Mouse Mitogen-activated protein kinase 15 ELISA Kit; Mapk15/MAP kinase 15/MAPK 15/Extracellular signal-regulated kinase 7/ERK-7; Mapk15 elisa kit

• Reactivity: Mouse
• Sequence Length: 544

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Mapk15 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Mapk15 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Mapk15 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Mapk15 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Mapk15 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 95147356
• NCBI GeneID: 225689
• NCBI Accession #: NP_620590.2
• NCBI GenBank Nucleotide #: NM_139021.2
• Molecular Weight: 31,112 Da
• NCBI Official Full Name: mitogen-activated protein kinase 15
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 15
• NCBI Official Symbol: MAPK15
• NCBI Official Synonym Symbols: ERK7; ERK8
• NCBI Protein Information: mitogen-activated protein kinase 15
• UniProt Protein Name: Mitogen-activated protein kinase 15
• Protein Family: Mitogen-activated protein kinase
• UniProt Gene Name: MAPK15
• UniProt Synonym Gene Names: ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
• UniProt Entry Name: MK15_HUMAN

Uniprot Description

ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.24; Protein kinase, CMGC; CMGC group; MAPK family; Erk7 subfamily

Chromosomal Location of Human Ortholog: -

Cellular Component: extracellular region; intracellular; nucleus

Molecular Function: ATP binding; MAP kinase activity; protein binding; SH3 domain binding

Biological Process: MAPKKK cascade; negative regulation of DNA replication; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of protein catabolic process; positive regulation of protein ubiquitination; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid autophosphorylation; response to estradiol stimulus