Goat Cyclooxygenase 2 ELISA Kit | COX2 elisa kit

Goat Cyclooxygenase 2 ELISA Kit

Cat. Num. AAA737051

• Gene Names: PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• Reactivity: Goat
• Synonyms: Cyclooxygenase 2; Goat Cyclooxygenase 2 ELISA Kit; COX2 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This COX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat COX2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COX2 elisa kit

Principle of the assay: COX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX2 antibody and an COX2-HRP conjugate. The assay sample and buffer are incubated together with COX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX2 concentration since COX2 from samples and COX2-HRP conjugate compete for the anti-COX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by COX2 from the sample, fewer sites are left to bind COX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COX2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 181254
• NCBI GeneID: 5743
• Molecular Weight: 68,996 Da
• NCBI Official Full Name: cyclooxygenase-2
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• NCBI Official Symbol: PTGS2
• NCBI Official Synonym Symbols: COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• NCBI Protein Information: prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2
• UniProt Protein Name: Prostaglandin G/H synthase 2
• UniProt Gene Name: PTGS2
• UniProt Synonym Gene Names: COX2; COX-2; PGH synthase 2; PGHS-2
• UniProt Entry Name: PGH2_HUMAN

NCBI Description

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Uniprot Description

COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.

Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.99.1

Chromosomal Location of Human Ortholog: 1q25.2-q25.3

Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus

Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; protein homodimerization activity; enzyme binding; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; decidualization; sensory perception of pain; prostaglandin biosynthetic process; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission, dopaminergic; response to estradiol stimulus; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; negative regulation of smooth muscle contraction; regulation of blood pressure; arachidonic acid metabolic process; positive regulation of smooth muscle contraction; angiogenesis; inflammatory response; water-soluble vitamin metabolic process; response to fructose stimulus; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; hair cycle; prostaglandin metabolic process; response to manganese ion; ovulation; NAD metabolic process; regulation of inflammatory response; positive regulation of fever; positive regulation of vasoconstriction; brown fat cell differentiation; response to oxidative stress; cell motility; embryo implantation