Canine Apolipoprotein C2 precursor ELISA Kit | Pre-APO-C2 elisa kit

Canine Apolipoprotein C2 precursor ELISA Kit

Cat. Num. AAA744551

• Gene Names: APOC2; APO-CII; APOC-II
• Reactivity: Canine
• Synonyms: Apolipoprotein C2 precursor; Canine Apolipoprotein C2 precursor ELISA Kit; Pre-APO-C2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ApoC2p ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ApoC2p. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This ApoC2p ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ApoC2p. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Pre-APO-C2 elisa kit

Principle of the Assay: ApoC2p ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoC2p antibody and an ApoC2p-HRP conjugate. The assay sample and buffer are incubated together with ApoC2p-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoC2p concentration since ApoC2p from samples and ApoC2p-HRP conjugate compete for the anti-ApoC2p antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoC2p from the sample, fewer sites are left to bind ApoC2p-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoC2p concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Pre-APO-C2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 32130518
• NCBI GeneID: 344
• NCBI Accession #: NP_000474.2
• NCBI GenBank Nucleotide #: NM_000483.4
• Molecular Weight: 11,284 Da
• NCBI Official Full Name: apolipoprotein C-II
• NCBI Official Synonym Full Names: apolipoprotein C-II
• NCBI Official Symbol: APOC2
• NCBI Official Synonym Symbols: APO-CII; APOC-II
• NCBI Protein Information: apolipoprotein C-II; apolipoprotein C2
• UniProt Protein Name: Apolipoprotein C-II
• UniProt Gene Name: APOC2
• UniProt Synonym Gene Names: APC2; Apo-CII; ApoC-II
• UniProt Entry Name: APOC2_HUMAN

NCBI Description

This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. This gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally occurring read-through transcription exists between this gene and the neighboring upstream apolipoprotein C-IV (APOC4) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

APOC2: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly. Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B). It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Belongs to the apolipoprotein C2 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: protein homodimerization activity; phospholipase binding; lipase inhibitor activity; lipid binding; phospholipase activator activity

Biological Process: phototransduction, visible light; positive regulation of fatty acid biosynthetic process; negative regulation of cholesterol transport; lipoprotein metabolic process; cholesterol efflux; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; negative regulation of catalytic activity; positive regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; retinoid metabolic process; negative regulation of lipid metabolic process; lipid catabolic process

Disease: Apolipoprotein C-ii Deficiency