Rabbit anti-Mouse, Human Hspa5 Polyclonal Antibody | anti-Hspa5 antibody

Hspa5 Antibody

Cat. Num. AAA7045622

• Gene Names: Hspa5; Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
• Reactivity: Mouse, Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: Hspa5; Polyclonal Antibody; Hspa5 Antibody; 78 kDa glucose-regulated protein; GRP-78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; Grp78; anti-Hspa5 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 655

• Applicable Applications for anti-Hspa5 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Species: Mouse
• Immunogen: Recombinant mouse 78 kDa glucose-regulated protein (20-655AA)
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat liver tissue,Rat brain tissue,Mouse brain tissueAll lanes: Hspa5 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73 KDaObserved band size: 73 KDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG-2 cells using MBS7045622 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-Hspa5 antibody

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 254540168
• NCBI GeneID: 14828
• NCBI Accession #: NP_001156906.1
• NCBI GenBank Nucleotide #: NM_001163434.1
• UniProt Accession #: P20029; O35642; Q3UFF2; Q61630
• Molecular Weight: 72,422 Da
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock protein 5
• NCBI Official Symbol: Hspa5
• NCBI Official Synonym Symbols: Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: Hspa5
• UniProt Synonym Gene Names: Grp78; GRP-78; BiP
• UniProt Entry Name: GRP78_MOUSE

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; focal adhesion; integral to endoplasmic reticulum membrane; membrane; midbody; mitochondrion; myelin sheath; nucleus; plasma membrane; signalosome; smooth endoplasmic reticulum

Molecular Function: ATPase activity; enzyme binding; glycoprotein binding; misfolded protein binding; protein binding; protein domain specific binding; ribosome binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: cellular response to glucose starvation; cerebellar Purkinje cell layer development; cerebellum structural organization; ER overload response; negative regulation of apoptosis; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; positive regulation of embryonic development; positive regulation of protein ubiquitination; proteolysis involved in cellular protein catabolic process; unfolded protein response, activation of signaling protein activity