SDS-PAGE
PPP3CA / phosphatase 2B Recombinant Protein | PPP3CA recombinant protein
Recombinant Human PPP3CA / Protein phosphatase 2B Protein (His tag)
Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
Purity
> 94 % as determined by SDS-PAGE
Synonyms
PPP3CA / phosphatase 2B; Recombinant Human PPP3CA / Protein phosphatase 2B Protein (His tag); PPP3CA; CALNA; CAN; PPP3CA recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
> 94 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
Sequence Length
521
Application Notes
The recombinant human PPP3CA consists of 538 amino acids and has a calculated molecular mass of 60.8 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
His
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for PPP3CA recombinant protein
Background: PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.
Description: A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.
Description: A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,245 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
NCBI Official Synonym Full Names
protein phosphatase 3 catalytic subunit alpha
NCBI Official Symbol
PPP3CA
NCBI Official Synonym Symbols
CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
NCBI Protein Information
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Protein Family
UniProt Gene Name
PPP3CA
UniProt Synonym Gene Names
CALNA; CNA
Uniprot Description
PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .
Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: calcineurin complex; cytoplasm; cytosol; nucleoplasm
Molecular Function: calmodulin binding; calmodulin-dependent protein phosphatase activity; drug binding; enzyme binding; protein binding; protein dimerization activity
Biological Process: calcineurin-NFAT signaling pathway; dephosphorylation; positive regulation of NFAT protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; response to calcium ion; T cell activation; Wnt receptor signaling pathway, calcium modulating pathway
Research Articles on PPP3CA
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Product Notes
The PPP3CA ppp3ca (Catalog #AAA2545262) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human PPP3CA consists of 538 amino acids and has a calculated molecular mass of 60.8 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the PPP3CA ppp3ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP3CA / phosphatase 2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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