Calcineurin A/PPP3CA Active Protein | PPP3CA active protein

Calcineurin A/PPP3CA Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121504

• Gene Names: PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• Purity: >94% as determined by SDS-PAGE
• Synonyms: Calcineurin A/PPP3CA; Calcineurin A/PPP3CA Protein; Human; Recombinant (His Tag); Human PPP3CA/Protein phosphatase 2B Protein (His Tag); CALN Protein; CALNA Protein; CALNA1 Protein; CCN1 Protein; CNA1 Protein; PPP2B Protein; protein phosphatase 3; catalytic subunit; alpha isozyme; PPP3CA active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser2-Gln521

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PPP3CA (Q08209-1)(Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Using the Octet RED System. The affinity constant (Kd) of human PPPP3CA-His bound to Human PPIA-His was 0.9nM.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PPP3CA active protein

Background: PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.

Product Categories/Family for PPP3CA active protein

Calcium-binding Proteins and Related Molecules; Non-Canonical Wnt Pathway

References

Frey N, et al. (2002) Calsarcin-3, a Novel Skeletal Muscle-specific Member of the Calsarcin Family, Interacts with Multiple Z-disc Proteins. Journal of Biological Chemistry. 277(16): 13998-4004.Frey N, et al. (2000) Calsarcins, a novel family of sarcomeric calcineurin-binding proteins. Proceedings of the National Academy of Sciences. 97(26):14632-7.Crabtree G R, et al. (1999) Generic signals and specific outcomes: Signaling through Ca2+, calcineurin, and NF-AT. Cell. 96(5):611-4.

NCBI and Uniprot Product Information

• NCBI GI #: 1352673
• NCBI GeneID: 5530
• UniProt Accession #: Q08209; Q8TAW9; A1A441; A8K3B7; A8W6Z7; A8W6Z8; B5BUA2
• Molecular Weight: 521
• NCBI Official Full Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP3CA
• NCBI Official Synonym Symbols: CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatas
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP3CA
• UniProt Synonym Gene Names: CALNA; CNA
• UniProt Entry Name: PP2BA_HUMAN

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; Z disc; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein dimerization activity; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; protein serine/threonine phosphatase activity; drug binding; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; calcineurin-NFAT signaling pathway; transition between fast and slow fiber; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle