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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RPL30 antibody at 1:3000 dilution.)

Rabbit RPL30 Polyclonal Antibody | anti-RPL30 antibody

RPL30 Polyclonal Antibody

Gene Names
RPL30; L30
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
RPL30; Polyclonal Antibody; RPL30 Polyclonal Antibody; L30; anti-RPL30 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 71% glycerol.
PBS with 0.02% sodium azide, 50% glycerol, pH7.24
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RPL30 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:50-1:100
Immunogen
Recombinant fusion protein of human RPL30 (NP_000980.1)
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RPL30 antibody at 1:3000 dilution.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RPL30 antibody at 1:3000 dilution.)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis of HeLa cells using RPL30 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Confocal immunofluorescence analysis of HeLa cells using RPL30 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat heart using RPL30 antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat heart using RPL30 antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using RPL30 antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon carcinoma using RPL30 antibody at dilution of 1:100 .)
Related Product Information for anti-RPL30 antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Product Categories/Family for anti-RPL30 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,784 Da
NCBI Official Full Name
60S ribosomal protein L30
NCBI Official Synonym Full Names
ribosomal protein L30
NCBI Official Symbol
RPL30
NCBI Official Synonym Symbols
L30
NCBI Protein Information
60S ribosomal protein L30
UniProt Protein Name
60S ribosomal protein L30
Protein Family
UniProt Gene Name
RPL30
UniProt Entry Name
RL30_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL30: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: focal adhesion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination

Research Articles on RPL30

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Product Notes

The RPL30 rpl30 (Catalog #AAA9457671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPL30 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPL30 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:100 IF: 1:50-1:100. Researchers should empirically determine the suitability of the RPL30 rpl30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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