Immunohistochemistry (IHC)
(MBS9606867 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit PPP3CA Polyclonal Antibody | anti-PPP3CA antibody
PPP3CA Antibody
Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; CALNA1; IECEE1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PPP3CA; Polyclonal Antibody; PPP3CA Antibody; Alpha isoform formerly PPP2B; Calcineurin A alpha; Calcineurin A1; CalcineurinA; Calmodulin dependent calcineurin A subunit alpha isoform; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA 1; CALNA; CALNA1; CAM PRP catalytic subunit; CAM-PRP catalytic subunit; CCN 1; CCN1; CNA 1; CNA alpha; CNA; CNA1; PP2BA_HUMAN; PPP2B; Ppp3ca; Protein phosphatase 2B catalytic subunit; Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform; Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA; Protein phosphatase 3 catalytic subunit alpha isozyme; Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; anti-PPP3CA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PPP3CA antibody detects endogenous levels of total PPP3CA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
521
Applicable Applications for anti-PPP3CA antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human PPP3CA
Subcellular Location
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
Predicted Cross Reactivity
Pig, Bovine, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9606867 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606867 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using PPP3CA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using PPP3CA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-PPP3CA antibody
Description: In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-A?, PP2B-Aˆ« and PP2B-A?. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2.
Function: Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
Subunit Structure: Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity (By similarity). Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3 (PubMed:11114196, PubMed:11842093). Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria (PubMed:18838687). Interacts with CMYA5; this interaction represses calcineurin activity in muscle (By similarity).Interacts with CIB1; the interaction increases upon cardiomyocytes hypertrophy (By similarity). Interacts with CHP1 and CHP2 (By similarity). Interacts (constitutively active form) with SYNPO2 (PubMed:17923693).
Similarity: Belongs to the PPP phosphatase family. PP-2B subfamily.
Function: Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
Subunit Structure: Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity (By similarity). Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3 (PubMed:11114196, PubMed:11842093). Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria (PubMed:18838687). Interacts with CMYA5; this interaction represses calcineurin activity in muscle (By similarity).Interacts with CIB1; the interaction increases upon cardiomyocytes hypertrophy (By similarity). Interacts with CHP1 and CHP2 (By similarity). Interacts (constitutively active form) with SYNPO2 (PubMed:17923693).
Similarity: Belongs to the PPP phosphatase family. PP-2B subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 61 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
NCBI Official Synonym Full Names
protein phosphatase 3 catalytic subunit alpha
NCBI Official Symbol
PPP3CA
NCBI Official Synonym Symbols
CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; CALNA1; IECEE1
NCBI Protein Information
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Protein Family
UniProt Gene Name
PPP3CA
Uniprot Description
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca2+-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722). In response to increased Ca2+ levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca2+ levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Dephosphorylates heat shock protein HSPB1 (). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca2+ levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).
Research Articles on PPP3CA
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Product Notes
The PPP3CA ppp3ca (Catalog #AAA9606867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP3CA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP3CA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PPP3CA ppp3ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP3CA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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