PPIL2 recombinant protein

PPIL2 Protein, Human, Recombinant (aa 280-457, His Tag)

Cat. Num. AAA8121684

• Gene Names: PPIL2; CYC4; CYP60; UBOX7; Cyp-60; hCyP-60
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PPIL2; PPIL2 Protein; Human; Recombinant (aa 280-457; His Tag); Human PPIL2 Protein (aa 280-457; CYC4 Protein; Cyp-60 Protein; CYP60 Protein; hCyP-60 Protein; UBOX7 Protein; peptidylprolyl isomerase (cyclophilin)-like 2; PPIL2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly280-Pro457

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human PPIL2 (Q13356-2)(Gly280-Pro457) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PPIL2 recombinant protein

Background: PPIL2, is an enzyme that belongs to the cyclophilin family. The cyclophilins are peptidylprolyl isomerases and are highly conserved ubiquitous. They play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL2 interacts with the proteinase inhibitor eglin c and is localized in the nucleus. It contains 1 PPIL2 cyclophilin-type domain and 1 U-box domain. PPIL2 accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

References

Grouse LH. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903. Lennon G. et al., 1997, Genome Res. 6 (9): 791-806. Payan DG. et al., 1996, Biochem J. 314 (1): 313-9.

NCBI and Uniprot Product Information

• NCBI GI #: 7657473
• NCBI GeneID: 23759
• NCBI Accession #: NP_055152.1
• NCBI GenBank Nucleotide #: NM_014337.3
• UniProt Accession #: Q13356; Q13357; Q8TAH2; Q9BWR8
• Molecular Weight: 59,458 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase-like 2 isoform a
• NCBI Official Synonym Full Names: peptidylprolyl isomerase (cyclophilin)-like 2
• NCBI Official Symbol: PPIL2
• NCBI Official Synonym Symbols: CYC4; CYP60; UBOX7; Cyp-60; hCyP-60
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase-like 2; PPIase; U-box domain containing 7; cyclophilin, 60kDa; cyclophilin-60; cyclophilin-like protein CyP-60; peptidylprolyl cis-trans isomerase; rotamase PPIL2
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase-like 2
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PPIL2
• UniProt Synonym Gene Names: PPIaseCurated
• UniProt Entry Name: PPIL2_HUMAN

NCBI Description

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIL2: a peptidylprolyl isomerase (PPIase) of the cyclophilin-type family. PPIases accelerate the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds. Two alternatively-spliced isoforms have been described.

Protein type: Ligase; Cyclophilin; Ubiquitin conjugating system; EC 5.2.1.8; Spliceosome; Ubiquitin ligase; Isomerase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; Golgi lumen; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; ligase activity

Biological Process: protein polyubiquitination; protein peptidyl-prolyl isomerization; protein folding; blood coagulation; leukocyte migration