Immunohistochemistry (IHC)-Paraffin
(Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.)
Mouse anti-Human PAG1 Antibody | anti-PAG1 antibody
Anti-PAG1 Purified
Gene Names
Pag1; Cbp; Pag; F730007C19Rik
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
PAG1; Anti-PAG1 Purified; phosphoprotein membrane anchor with glycosphingoli; CBP; PAG; anti-PAG1 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Clone Number
MEM-255
Specificity
The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
429
Applicable Applications for anti-PAG1 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Negative Species
Mouse, Rat, Cow
Preparation and Storage
Store at 2-8 degree C.
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.)
Related Product Information for anti-PAG1 antibody
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
NCBI Official Synonym Full Names
phosphoprotein associated with glycosphingolipid microdomains 1
NCBI Official Symbol
Pag1
NCBI Official Synonym Symbols
Cbp; Pag; F730007C19Rik
NCBI Protein Information
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
UniProt Protein Name
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Protein Family
UniProt Gene Name
Pag1
UniProt Synonym Gene Names
Cbp; Pag
UniProt Entry Name
PHAG1_MOUSE
Research Articles on PAG1
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Product Notes
The PAG1 pag1 (Catalog #AAA1801159) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAG1 Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAG1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the PAG1 pag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAG1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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