Western Blot (WB)
(Anti-PPIL1 mouse monoclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.))
Mouse anti-Human PPIL1 Monoclonal Antibody | anti-PPIL1 antibody
Anti-PPIL1 Antibody, Mouse Monoclonal
No cross-reactivity in ELISA with E Coli cell lysate
ELISA: 1:1000-1:2000
FC/FACS/FCM: 1:25-1:100
IP: 4-6uL/mg of lysate
Western Blot (WB)
(Anti-PPIL1 mouse monoclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PPIL1 mouse monoclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(PPIL1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)
Immunoprecipitation (IP)
(PPIL1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq, Jul 2008]
Uniprot Description
PPIL1: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
Protein type: Cyclophilin; Isomerase; EC 5.2.1.8; Spliceosome
Chromosomal Location of Human Ortholog: 6p21.1
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity
Biological Process: nuclear mRNA splicing, via spliceosome; protein peptidyl-prolyl isomerization; protein folding
Research Articles on PPIL1
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Product Notes
The PPIL1 ppil1 (Catalog #AAA8107248) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPIL1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPIL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS/FCM), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:1000-1:2000 FC/FACS/FCM: 1:25-1:100 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the PPIL1 ppil1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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