SDS-Page
PPCDC recombinant protein
PPCDC Protein, Human, Recombinant (His Tag)
Purity
>95% as determined by SDS-PAGE
Synonyms
PPCDC; PPCDC Protein; Human; Recombinant (His Tag); Human PPC-DC/PPCDC Protein (His Tag); coaC Protein; MDS018 Protein; PPC-DC Protein; phosphopantothenoylcysteine decarboxylase; PPCDC recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, 15% Glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser204
Species
Human
Predicted N Terminal
His
Tag
N-His
Protein Construction
A DNA sequence encoding the mature form of human PPCDC (AAH14409.1)(Met1-Ser204) was expressed with a polyhistidine tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for PPCDC recombinant protein
Background: PPC-DC, also known as PPCDC, belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily which takes a part in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. This process include several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4''-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPC-DC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine.
References
Zody MC. et al., 2006, Nature. 440: 671-5. Ota T. et al., 2004, Nat Genet. 36: 40-5. Clark HF. et al., 2003, Genome Res. 13: 2265-70.
NCBI and Uniprot Product Information
Molecular Weight
The recombinant human PPCDC consists of 219 amino acids and predicts a molecular mass of 24.3kDa. It migrates as an approximately 24kDa band in SDS-PAGE under reducing conditions.
Protein Family
Similar Products
Product Notes
The PPCDC (Catalog #AAA8121735) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser20 4. It is sometimes possible for the material contained within the vial of "PPCDC, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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