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Western Blot (WB) (PPCDC Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PPCDC antibody detected the PPCDC protein (arrow).)

Rabbit anti-Mouse PPCDC Polyclonal Antibody | anti-PPCDC antibody

PPCDC, NT (PPCDC, COAC, Phosphopantothenoylcysteine decarboxylase, CoaC) (APC)

Gene Names
PPCDC; MDS018
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PPCDC; Polyclonal Antibody; NT (PPCDC; COAC; Phosphopantothenoylcysteine decarboxylase; CoaC) (APC); CoaC; anti-PPCDC antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PPCDC antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PPCDC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids from the N-terminal region of human PPCDC.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPCDC Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PPCDC antibody detected the PPCDC protein (arrow).)

Western Blot (WB) (PPCDC Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PPCDC antibody detected the PPCDC protein (arrow).)
Related Product Information for anti-PPCDC antibody
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]).
Product Categories/Family for anti-PPCDC antibody
References
Strauss, E., et al. Biochemistry 43(49):15520-15533(2004). Manoj, N., et al. Acta Crystallogr. D Biol. Crystallogr. 59 (PT 10), 1762-1766 (2003): Daugherty, M., et al. J. Biol. Chem. 277(24):21431-21439(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,395 Da
NCBI Official Full Name
phosphopantothenoylcysteine decarboxylase
NCBI Official Synonym Full Names
phosphopantothenoylcysteine decarboxylase
NCBI Official Symbol
PPCDC
NCBI Official Synonym Symbols
MDS018
NCBI Protein Information
phosphopantothenoylcysteine decarboxylase; coaC; PPC-DC
UniProt Protein Name
Phosphopantothenoylcysteine decarboxylase
UniProt Gene Name
PPCDC
UniProt Synonym Gene Names
COAC; PPC-DC
UniProt Entry Name
COAC_HUMAN

NCBI Description

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]

Uniprot Description

PPCDC: Necessary for the biosynthesis of coenzyme A. Catalyzes the decarboxylation of 4-phosphopantothenoylcysteine to form 4'- phosphopantotheine. Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.1.1.36; Lyase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: cytosol

Molecular Function: phosphopantothenoylcysteine decarboxylase activity

Biological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process; vitamin metabolic process; pantothenate metabolic process; water-soluble vitamin metabolic process

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Product Notes

The PPCDC ppcdc (Catalog #AAA6336171) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPCDC, NT (PPCDC, COAC, Phosphopantothenoylcysteine decarboxylase, CoaC) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPCDC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPCDC ppcdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPCDC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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