Porcine Haptoglobin (Hpt) RTU ELISA Kit | Hpt rtu elisa kit

Porcine Haptoglobin (Hpt) ELISA Kit

Cat. Num. AAA459906

• Reactivity: Porcine
• Synonyms: Haptoglobin (Hpt); Porcine Haptoglobin (Hpt) ELISA Kit; HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt rtu elisa kit

• Reactivity: Porcine
• Sequence Length: 347

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 78.1-5000ng/mL
• Sensitivity: 25.5ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hpt rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47522826
• NCBI GeneID: 397061
• NCBI Accession #: NP_999165.1
• NCBI GenBank Nucleotide #: NM_214000.2
• UniProt Accession #: Q8SPS7
• Molecular Weight: 38,482 Da
• NCBI Official Full Name: haptoglobin
• NCBI Official Symbol: HP
• NCBI Protein Information: haptoglobin
• UniProt Protein Name: Haptoglobin
• Protein Family: Hypoxanthine phosphoribosyltransferase
• UniProt Gene Name: HP

Uniprot Description

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway ().