Typical Testing Data/Standard Curve (for reference only)
Rat Claudin 5 (CLDN5) ELISA Kit | CLDN5 elisa kit
Rat Claudin 5 (CLDN5) ELISA Kit
Gene Names
CLDN5; AWAL; BEC1; TMVCF; CPETRL1
Reactivity
Rat
Synonyms
Claudin 5 (CLDN5); Rat Claudin 5 (CLDN5) ELISA Kit; AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS; CLDN5 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CLDN5. No significant cross-reactivity or interference between CLDN5 and analogues was observed.
Sequence Length
218
Samples
Rat tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.118ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLDN5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLDN5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CLDN5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CLDN5 in rat tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CLDN5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLDN5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CLDN5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLDN5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for CLDN5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,147 Da
NCBI Official Full Name
claudin-5
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
CLDN5
NCBI Official Synonym Symbols
AWAL; BEC1; TMVCF; CPETRL1
NCBI Protein Information
claudin-5
UniProt Protein Name
Claudin-5
Protein Family
UniProt Gene Name
CLDN5
UniProt Synonym Gene Names
AWAL; TMVCF; TMDVCF
UniProt Entry Name
CLD5_HUMAN
NCBI Description
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]
Uniprot Description
Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: tight junction; integral to membrane; plasma membrane; intercellular junction
Molecular Function: identical protein binding; structural molecule activity
Biological Process: calcium-independent cell-cell adhesion
Research Articles on CLDN5
Similar Products
Product Notes
The Rat CLDN5 cldn5 (Catalog #AAA2022331) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2022331 ELISA Kit recognizes Rat CLDN5. It is sometimes possible for the material contained within the vial of "Claudin 5 (CLDN5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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