Rabbit anti-Human PSKH1 Polyclonal Antibody | anti-PSKH1 antibody

PSKH1, NT (Protein Serine Kinase H1, PSK-H1, Serine/threonine Protein Kinase H1) (PE)

Cat. Num. AAA6495978

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PSKH1; Polyclonal Antibody; NT (Protein Serine Kinase H1; PSK-H1; Serine/threonine Protein Kinase H1) (PE); EC=2.7.11.1; anti-PSKH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSKH1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PSKH1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PSKH1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PSKH1 Antibody (N-term H70) in Hela cell line lysate and mouse kidney tissue lysate (35ug/lane). PSKH1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PSKH1 antibody

Antibodies; Protein Kinases

References

1. Brede, G., et al., Exp. Cell Res. 291(2):299-312 (2003). 2. Brede, G., et al., Nucleic Acids Res. 30(23):5301-5309 (2002). 3. Brede, G., et al., Genomics 70(1):82-92 (2000). 4. Amarzguioui, M., et al., Nucleic Acids Res. 28(21):4113-4124 (2000). 5. Larsen, F., et al., Hum. Mol. Genet. 2(10):1589-1595 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 27901803
• NCBI GeneID: 5681
• NCBI Accession #: NP_006733
• NCBI GenBank Nucleotide #: NM_006742
• UniProt Accession #: P11801; Q9NY19
• Molecular Weight: 48,035 Da
• NCBI Official Full Name: serine/threonine-protein kinase H1
• NCBI Official Synonym Full Names: protein serine kinase H1
• NCBI Official Symbol: PSKH1
• NCBI Protein Information: serine/threonine-protein kinase H1; PSK-H1
• UniProt Protein Name: Serine/threonine-protein kinase H1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PSKH1
• UniProt Synonym Gene Names: PSK-H1
• UniProt Entry Name: KPSH1_HUMAN

Uniprot Description

PSKH1: May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; PSK family

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; microtubule organizing center; nuclear speck

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: protein amino acid phosphorylation