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Typical Testing Data/Standard Curve (for reference only)

Porcine Talin ELISA Kit | TLN1 elisa kit

Porcine Talin ELISA Kit

Gene Names
TLN1; TLN; ILWEQ
Reactivity
Porcine
Synonyms
Talin; Porcine Talin ELISA Kit; TLN1 elisa kit
Ordering
For Research Use Only!
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TLN1 elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This TLN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TLN. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY TLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLN antibody and an TLN-HRP conjugate. The assay sample and buffer are incubated together with TLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLN concentration since TLN from samples and TLN-HRP conjugate compete for the anti-TLN antibody binding site. Since the number of sites is limited, as more sites are occupied by TLN from the sample, fewer sites are left to bind TLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TLN1 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
269,767 Da
NCBI Official Full Name
talin
NCBI Official Synonym Full Names
talin 1
NCBI Official Symbol
TLN1
NCBI Official Synonym Symbols
TLN; ILWEQ
NCBI Protein Information
talin-1
UniProt Protein Name
Talin-1
Protein Family
UniProt Gene Name
TLN1
UniProt Synonym Gene Names
KIAA1027; TLN
UniProt Entry Name
TLN1_HUMAN

NCBI Description

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

Uniprot Description

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; plasma membrane; extracellular region; microtubule organizing center; intercellular junction; cytosol

Molecular Function: integrin binding; actin filament binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding

Biological Process: platelet activation; intercellular junction assembly; axon guidance; unfolded protein response; cytoskeletal anchoring; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; platelet degranulation; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; cell motility; blood coagulation

Research Articles on TLN1

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Product Notes

The Porcine TLN1 tln1 (Catalog #AAA741302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741302 ELISA Kit recognizes Porcine TLN1. It is sometimes possible for the material contained within the vial of "Talin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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