Goat AP-2 gamma Polyclonal Antibody | anti-TFAP2C antibody

Goat anti-AP-2 gamma Antibody

Cat. Num. AAA420258

• Gene Names: TFAP2C; ERF1; TFAP2G; hAP-2g; AP2-GAMMA
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Rat, Mouse, Dog, Pig, Cow
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: AP-2 gamma; Polyclonal Antibody; Goat anti-AP-2 gamma Antibody; TFAP2C; ERF1; TFAP2G; hAP-2g; AP2-GAMMA; transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma); estrogen receptor factor 1; transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma); TFAP2C antibody; ERF1 antibody; TFAP2G antibody; hAP-2g antibody; AP2-GAMMA antibody; transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) antibody; estrogen receptor factor 1 antibody; transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma) antibody; anti-TFAP2C antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Rat, Mouse, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NKTLEKMEKHRK
• Sequence Length: 450

• Applicable Applications for anti-TFAP2C antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Immunohistochemistry: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.; Western Blot: WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Immunogen: Peptide with sequence C-NKTLEKMEKHRK, from the C Terminus of the protein sequence according to NP_003213.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((4ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507445
• NCBI GeneID: 7022
• NCBI Accession #: NP_003213.1
• NCBI GenBank Nucleotide #: NM_003222.3
• Molecular Weight: 31,010 Da
• NCBI Official Full Name: transcription factor AP-2 gamma
• NCBI Official Synonym Full Names: transcription factor AP-2 gamma
• NCBI Official Symbol: TFAP2C
• NCBI Official Synonym Symbols: ERF1; TFAP2G; hAP-2g; AP2-GAMMA
• NCBI Protein Information: transcription factor AP-2 gamma
• UniProt Protein Name: Transcription factor AP-2 gamma
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2C
• UniProt Synonym Gene Names: AP2-gamma
• UniProt Entry Name: AP2C_HUMAN

NCBI Description

The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube. [provided by RefSeq, Jul 2008]

Uniprot Description

AP-2 gamma: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. During retinoic acid-mediated differentiation. Belongs to the AP-2 family.

Protein type: Motility/polarity/chemotaxis; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: mitochondrion; nucleoplasm; nucleus

Molecular Function: DNA binding; protein binding; protein dimerization activity; transcription factor activity

Biological Process: cell-cell signaling; male gonad development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; regulation of gene expression, epigenetic; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter